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Series GSE140617 Query DataSets for GSE140617
Status Public on Jun 18, 2020
Title Heat-shock molecular chaperone Hsp90 is involved in heterochromatin assembly in Schizosaccharomyces pombe.
Organism Schizosaccharomyces pombe
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Here, we report the high-throughput profiling of histone modification (H3K9me2) in fission yeast Schizosaccharomyces pombe. We generated genome-wide H3K9me2 maps of fission yeast mutants in swo1-26 (temperature sensitive, ts) cells at 25℃ and 37℃. We find that H3K9me2 enrichment at heterochromatin regions, especially at the mating-type locus and subtelomeres, is compromised, suggesting heterochromatin assembly defects.
Overall design Examination of H3K9me2 in wt, ago1D and tas3D growing at 25℃. Particularly, swo1-26 mutant cells are cultured under permissive condition (25℃) and non-permissive condition (25℃ shifted to 37℃ for 4 h).
Contributor(s) Li W, Sun L, Jin Q
Citation(s) 32499408
Submission date Nov 18, 2019
Last update date Sep 17, 2020
Contact name Li Sun
Organization name Xiamen University
Street address South Xiang'an Road
City Xiamen
State/province Fujian
ZIP/Postal code 361102
Country China
Platforms (1)
GPL22668 Ion Torrent PGM (Schizosaccharomyces pombe)
Samples (5)
GSM4175851 H3K9me2_wt_25
GSM4175852 H3K9me2_swo1-26_ts_25
GSM4175853 H3K9me2_swo1-26_ts_37
BioProject PRJNA590253
SRA SRP230449

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Supplementary file Size Download File type/resource
GSE140617_RAW.tar 3.4 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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