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| Status |
Public on Jan 31, 2020 |
| Title |
Tacrolimus- and sirolimus-induced human b cell dysfunction is reversible and preventable |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used RNA-seq to investigate transcriptional changes in human islet grafts that were treated in vivo with tacrolimus or sirolimus. We show that this treatment induces broad transcriptional dysregulation related to peptide processing, ion/calcium flux, and the extracellular matrix.
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| Overall design |
Human islets were tranplanted under kidney capsule of NSG mouse. After two weeks engraftment period, the mice were treated with tacrolimus, sirolimus, or PBS-control for 4 weeks. The grafts were then removed for analysis.
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| |
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| Contributor(s) |
Dai C, Walker JT, Prasad N, Powers AC |
| Citation(s) |
31941840 |
| |
| Submission date |
Nov 12, 2019 |
| Last update date |
May 01, 2020 |
| Contact name |
Alvin C Powers |
| E-mail(s) |
Al.Powers@vanderbilt.edu
|
| Phone |
6159367678
|
| Organization name |
Vanderbilt University
|
| Street address |
7465 MRBIV 2215 Garland Avenue
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (13)
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| Relations |
| BioProject |
PRJNA588996 |
| SRA |
SRP229589 |
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