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| Status |
Public on Jul 17, 2009 |
| Title |
Expression profiling of Bmal mutant dorsal skin at telogen of hair follicle cycling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Hair follicles undergo recurrent cycling of controlled growth (anagen), regression (catagen), and relative quiescence (telogen) with a defined periodicity. Taking a genomics approach to study gene expression during synchronized mouse hair follicle cycling, we discovered that, in addition to circadian fluctuation, CLOCK-regulated genes are also modulated in phase with the hair growth cycle. During telogen and early anagen, circadian clock genes are prominently expressed in the secondary hair germ, which contains precursor cells for the growing follicle. Analysis of Clock and Bmal1 mutant mice reveals a delay in anagen progression, and the secondary hair germ cells show decreased levels of phosphorylated Rb and lack mitotic cells, suggesting that circadian clock genes regulate anagen progression via their effect on the cell cycle. Consistent with a block at the G1 phase of the cell cycle, we show a significant upregulation of p21 in Bmal1 mutant skin. While circadian clock mechanisms have been implicated in a variety of diurnal biological processes, our findings indicate that circadian clock genes may be utilized to modulate the progression of non-diurnal cyclic processes.
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| Overall design |
To gain molecular understanding of the the hair cycle delay in Bmal mutant mice, we profiled the dorsal skin of Bmal knockout (-/-) and their heterozygous (+/-) littermates at P22. At P22, the skin samples are comparable because all the samples are in telogen just prior to the hair cycle delay was observed. Histological sections were used to classify each sample into specific stage of the hair growth cycle based on established morphological guidelines. RNA from each mouse dorsal skin were separately hybridized to an Affymetrix Mouse Gene 1.0 ST array.
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| Contributor(s) |
Lin KK, Kumar V, Geyfman M, Chudova D, Ihler AT, Smyth P, Paus R, Takahashi JS, Andersen B |
| Citation(s) |
19629164 |
| Submission date |
Dec 16, 2008 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Kevin K Lin |
| E-mail(s) |
kklin@alumni.uci.edu
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| Organization name |
University of California, Irvine
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| Department |
Biological Chemistry & Medicine
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| Lab |
Bogi Andersen
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| Street address |
250 Sprague Hall
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| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697-4030 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM351621 |
Expression profiling of Bmal +/- littermate dorsal skin_rep1 |
| GSM351623 |
Expression profiling of Bmal +/- littermate dorsal skin_rep2 |
| GSM351625 |
Expression profiling of Bmal +/- littermate dorsal skin_rep3 |
| GSM351639 |
Expression profiling of Bmal -/- littermate dorsal skin_rep1 |
| GSM351642 |
Expression profiling of Bmal -/- littermate dorsal skin_rep2 |
| GSM351643 |
Expression profiling of Bmal -/- littermate dorsal skin_rep3 |
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| Relations |
| BioProject |
PRJNA112465 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14006_RAW.tar |
28.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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