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| Status |
Public on Oct 04, 2022 |
| Title |
Effects of Different Doses of rAAV 5-mCherry on Transcriptome of Hippocampal Pyramidal Neurons in Male Mouse |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Here, we evaluated the effects of three different doses of rAAV5 vectors, which are widely used for functional studies in the brain, on hippocampal pyramidal neurons transcription in male mice.
Results: There was a strong correlation between rAAV dose and toxicity. As dose increased, rAAV5-DIO-mCherry upregulated genes related to the immune and inflammation response and neuron death and downregulated genes associated with the synapse and axon compartments, mitochondrial potein complex suggesting that higher dose rAAV 5-DIO-mCherry may induce pyramidal neuronal fragility and impair synaptic transmission and structure.
Conclusions:the dose should be taken into consideration when rAAV vectors are used for gene transduction studies.
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| Overall design |
nine samples were analyzed. there three groups,and three biological replication for each group
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| Contributor(s) |
Liu Y, Chen Y, Li Z, chen H, Hu N, Yang J, Gao T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 06, 2019 |
| Last update date |
Oct 06, 2022 |
| Contact name |
YISI LIU |
| E-mail(s) |
1004371737@qq.com
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| Organization name |
SOUTH MEDICAL UNIVERSITY
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| Street address |
SHATAI ROAD
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| City |
GUANGZHOU |
| ZIP/Postal code |
510000 |
| Country |
China |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA587942 |
| SRA |
SRP228768 |
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