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Series GSE139888 Query DataSets for GSE139888
Status Public on Nov 05, 2019
Title Cell specific profiling of neurons expressing differnet neurotransmitters during Drosophila development
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In order to gain a greater understanding of neurotransmitter specification we profiled the transcription state of cholinergic, GABAergic and glutamatergic neurons in vivo at multiple developmental time points. We identified 86 differentially expressed transcription factors that are uniquely enriched, or uniquely depleted, in a specific neurotransmitter subtype. Some transcription factors show a similar profile across development, others only show enrichment or depletion at specific developmental stages. Profiling of acj6 (cholinergic enriched) and Ets65A (cholinergic depleted) binding sites in vivo reveals that they both directly bind the ChAT locus, in addition to a wide spectrum of other key neuronal differentiation genes.
 
Overall design Profiling of RNA Polymerase II occupancy in different neurotransmitter populations of neurons in Drosophila, at different developmental stages. Also profiling of acj6 and Ets65A-RA genomic binding in the Drosophila brain.
 
Contributor(s) Southall TD, Estacio-Gómez A
Citation(s) 32493733
Submission date Nov 04, 2019
Last update date Jun 22, 2020
Contact name Tony David Southall
E-mail(s) t.southall@imperial.ac.uk
Organization name Imperial College London
Department Life Sciences
Lab Southall lab
Street address South Kensington Campus
City London
ZIP/Postal code SW7 2AZ
Country United Kingdom
 
Platforms (1)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
Samples (31)
GSM4148221 emb_ChAT_Dam-POLII_1: RNA Pol II occupancy in embryonic cholinergic neurons
GSM4148222 emb_ChAT_Dam-POLII_2: RNA Pol II occupancy in embryonic cholinergic neurons
GSM4148223 emb_ChAT_Dam-POLII_3: RNA Pol II occupancy in embryonic cholinergic neurons
Relations
BioProject PRJNA587481
SRA SRP228543

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Supplementary file Size Download File type/resource
GSE139888_RAW.tar 147.7 Mb (http)(custom) TAR (of GFF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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