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| Status |
Public on Oct 21, 2020 |
| Title |
Transcriptome-wide identification of nuclear and cytoplasmic RNA-binding sites of FUS RBD-only constructs |
| Organism |
Homo sapiens |
| Experiment type |
Other
Expression profiling by high throughput sequencing
|
| Summary |
We report the binding sites of three FUS constructs: (1) The full-length protein (FL); (2) A deletion mutant consisting of amino acids 242-526 (CT); and (3) A aa 242-526 deletion mutant with ALS-associated P525L mutation and heterologous nuclear export signal (CTstar) with the aim to assess the role of protein-protein interactions on RNA-binding and to identify nuclear and cytoplasmic RNA-binding sites of Fused in Sarcoma (FUS).
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| |
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| Overall design |
Three HITS-CLIP experiments were performed in triplicate each. Input RNA was sequenced after depletion of ribosomal RNA.
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| |
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| Contributor(s) |
Jutzi D, Ruepp M |
| Citation(s) |
33311468 |
| |
| Submission date |
Oct 22, 2019 |
| Last update date |
Dec 22, 2020 |
| Contact name |
Marc-David Ruepp |
| Organization name |
King's College
|
| Department |
Dementia Research Institute
|
| Street address |
5 Cutcombe Road
|
| City |
London |
| ZIP/Postal code |
SE5 9RT |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA578929 |
| SRA |
SRP226638 |
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