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Series GSE139263 Query DataSets for GSE139263
Status Public on Oct 21, 2020
Title Transcriptome-wide identification of nuclear and cytoplasmic RNA-binding sites of FUS RBD-only constructs
Organism Homo sapiens
Experiment type Other
Expression profiling by high throughput sequencing
Summary We report the binding sites of three FUS constructs: (1) The full-length protein (FL); (2) A deletion mutant consisting of amino acids 242-526 (CT); and (3) A aa 242-526 deletion mutant with ALS-associated P525L mutation and heterologous nuclear export signal (CTstar) with the aim to assess the role of protein-protein interactions on RNA-binding and to identify nuclear and cytoplasmic RNA-binding sites of Fused in Sarcoma (FUS).
Overall design Three HITS-CLIP experiments were performed in triplicate each. Input RNA was sequenced after depletion of ribosomal RNA.
Contributor(s) Jutzi D, Ruepp M
Citation(s) 33311468
Submission date Oct 22, 2019
Last update date Dec 22, 2020
Contact name Marc-David Ruepp
Organization name King's College
Department Dementia Research Institute
Street address 5 Cutcombe Road
City London
ZIP/Postal code SE5 9RT
Country United Kingdom
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (12)
GSM4134988 FL FUS CLIP-Seq rep 1
GSM4134989 FL FUS CLIP-Seq rep 2
GSM4134990 FL FUS CLIP-Seq rep 3
BioProject PRJNA578929
SRA SRP226638

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139263_RAW.tar 12.2 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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