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| Status |
Public on Jul 01, 2020 |
| Title |
Timeless collaborates with DDX11 to ensure processive replication of G4-forming DNA [RNA-seq] |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Regions of DNA with the potential to form secondary structure pose a frequent and significant impediment to DNA replication that must be actively countered by cells in order to preserve genetic and epigenetic integrity. The fork protection complex (FPC), a conserved group of replisome-associated proteins, comprising Timeless, plays an important role in maintaining efficient replisome function. A previously unappreciated DNA binding domain in the C terminus of Timeless, which exhibits specificity towards G4 structures, acts in collaboration with the DDX11 helicase to ensure replication of G4 structures in vivo and maintenance of genetic and epigenetic stability. Using RNA-seq experiments, we show that (i) Timeless and DDX11 share common gene targets, (ii) the promoter of Timeless- and DDX11-dependent genes are enriched in G4 structures in the vicinity of their promoter and (iii) both Timeless and DDX11 share a common set of gene targets with the FANCJ helicase known to maintain epigenetic stability in the vicinity of G4 structures.
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| Overall design |
RNA-seq data of WT, TIMELESS, DDX11 and FANCJ KO DT40 cells
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| |
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| Contributor(s) |
Koch Lerner L, Murat P, Sale JE |
| Citation(s) |
32705708 |
| |
| Submission date |
Oct 22, 2019 |
| Last update date |
Oct 05, 2020 |
| Contact name |
Julian E Sale |
| E-mail(s) |
jes@mrc-lmb.cam.ac.uk
|
| Organization name |
MRC Laboratory of Molecular Biology
|
| Department |
PNAC
|
| Street address |
Cambridge Biomedical Campus, Francis Crick Ave
|
| City |
Cambridge UK |
| ZIP/Postal code |
CB2 0QH |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL23499 |
Illumina HiSeq 4000 (Gallus gallus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA578917 |
| SRA |
SRP226629 |
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