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Series GSE139212 Query DataSets for GSE139212
Status Public on Oct 22, 2019
Title Neuronal transcriptome analyses reveal novel neuropeptide modulators of excitation and inhibition imbalance in C. elegans
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary Neuropeptides are secreted molecules that have conserved roles modulating many processes, including mood, reproduction, and feeding. Dysregulation of neuropeptide signaling is also implicated in neurological disorders such as epilepsy. However, much is unknown about the mechanisms regulating specific neuropeptides to mediate behavior. Here, we report that the expression levels of dozens of neuropeptides are up-regulated in response to circuit activity imbalance in C. elegans. acr-2 encodes a homolog of human nicotinic receptors, and functions in the cholinergic motoneurons. A hyperactive mutation, acr-2(gf), causes an activity imbalance in the motor circuit. We performed cell-type specific transcriptomic analysis and identified genes differentially expressed in acr-2(gf), compared to wild type. The most over-represented class of genes are neuropeptides, with insulin-like-peptides (ILPs) the most affected. Moreover, up-regulation of neuropeptides occurs in motor neurons, as well as sensory neurons. In particular, the induced expression of the ILP ins-29 occurs in the BAG neurons, which are previously shown to function in gas-sensing. We also show that this up-regulation of ins-29 in acr-2(gf) animals is activity-dependent. Our genetic and molecular analyses support cooperative effects for ILPs and other neuropeptides in promoting motor circuit activity in the acr-2(gf) background. Together, this data reveals that a major transcriptional response to motor circuit dysregulation is in up-regulation of multiple neuropeptides, and suggests that BAG sensory neurons can respond to intrinsic activity states to feedback on the motor circuit.
 
Overall design 4 total samples. Two replicates each of isolated cholinergic neurons from either wild type(strain CZ631 control) or acr-2(gf)(strain CZ5808 experimental) strain in C. elegans. Syncronized populations were collected at 72hr.
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Authors state "We did not include FPKM data for CZ5808 in our manuscript, but compared the sequence to CZ631 data in DESeq2. The FPKM data for CZ631 was just used as a comparison to previous studies."
 
Contributor(s) McCulloch KA, Zhou K, Jin Y
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 21, 2019
Last update date Oct 22, 2019
Contact name Yishi Jin
E-mail(s) yijin@ucsd.edu
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL22765 Illumina HiSeq 4000 (Caenorhabditis elegans)
Samples (4)
GSM4134098 Wild type cholinergic neurons adult replicate 1
GSM4134099 Wild type cholinergic neurons adult replicate 2
GSM4134100 acr-2(gf) cholinergic neurons adult replicate 1
Relations
BioProject PRJNA578749
SRA SRP226515

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Supplementary file Size Download File type/resource
GSE139212_DeSeq2.xlsx 2.5 Mb (ftp)(http) XLSX
GSE139212_Galaxy120-_Cufflinks_on_CZ631-1_gene_expression_.tabular.txt.gz 1.0 Mb (ftp)(http) TXT
GSE139212_Galaxy125-_Cufflinks_on_CZ631-2_gene_expression_.tabular.txt.gz 1.1 Mb (ftp)(http) TXT
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Processed data are available on Series record

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