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| Status |
Public on Oct 16, 2020 |
| Title |
A novel cardiomyogenic role for Isl1+ neural crest cells in the inflow tract |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study was to analyze the cellular compsitions of the arterial pole and venous pole regions of the neonatal mouse heart using single-cell RNA sequencing.
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| Overall design |
The dorsal and ventral domains of the cardiac base were dissected from 3-day old C57Bl6 male and female neonatal mice (n=15) and enzymatically dissociated into single-cell suspensions. The ventral domain included the proximal aorta, pulmonary artery, and outflow tract base. The dorsal domain included the sinoatrial node, cardiac ganglia and pulmonary veins. Single-cell preparations from 15 neonatal hearts were combined to generate two pools of dorsal and ventral cells, and cultured in vitro for 5 days to allow recovery from enzymatic and mechanical stress. Subsequently, cells were processed for 3' single cell RNA-seq using the 10X Genomics Chromium platform and sequenced on Illumina Novaseq 6000.
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| Contributor(s) |
Durante MA, Chatzistergos K |
| Citation(s) |
33268364 |
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| Submission date |
Oct 21, 2019 |
| Last update date |
Dec 09, 2020 |
| Contact name |
Michael Durante |
| E-mail(s) |
mdurante@med.miami.edu
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| Phone |
2393575338
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| Organization name |
University of Miami Miller School of Medicine
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| Department |
Ophthalmology
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| Lab |
Harbour
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| Street address |
1501 NW 10th Ave., Ste 832
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| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA578689 |
| SRA |
SRP226460 |
