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Series GSE139125 Query DataSets for GSE139125
Status Public on Oct 21, 2019
Title Single Cell RNA sequencing of mouse CD11b+, Gr1+ cells from the spleen from FVB/n and PYMT mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Myeloid-derived suppressor cells (MDSCs) are innate immune cells that acquire the capacity to suppress adaptive immune responses during cancer. It remains elusive how MDSCs differ from their normal myeloid counterparts, which limits our ability to specifically detect and therapeutically target MDSCs during cancer. Here, we used single-cell RNAseq to compare MDSC-containing splenic myeloid cells from breast tumor-bearing mice to wildtype controls. Our computational analysis of 14,646 single-cell transcriptomes reveals that MDSCs emerge through a previously unrealized aberrant neutrophil maturation trajectory in the spleen giving rise to a unique chemokine-responsive, immunosuppressive cell state that strongly differs from normal myeloid cells. We establish the first MDSC-specific gene signature and identify novel surface markers for improved detection and enrichment of MDSCs in murine and human samples. Our study provides a single-cell transcriptional map defining the development of MDSCs, which will ultimately enable us to specifically target these cells in cancer patients.
 
Overall design Droplet Based Single Cell RNA sequencing libraries were generated for 5 pooled FVB/n mouse spleens and 3 pooled PYMT mouse spleens using the 10x Genomics Chromium Platform and sequenced on the Illumina HighSeq 4000
 
Contributor(s) Alshetaiwi H, Pervolarakis N, Kessenbrock K
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Submission date Oct 20, 2019
Last update date Oct 23, 2019
Contact name Kai Kessenbrock
E-mail(s) kessenbrocklab@gmail.com
Phone 9498243424
Organization name UC Irvine
Department Biological Chemistry
Street address 839 Health Sciences Road
City Irvine
State/province CA
ZIP/Postal code 92617
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (2)
GSM4131336 Wild Type
GSM4131337 PYMT
Relations
BioProject PRJNA578550
SRA SRP226381

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139125_RAW.tar 25.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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