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| Status |
Public on Apr 23, 2020 |
| Title |
TET1 and 5-hydroxymethylation preserve the stem cell state of mouse trophoblast |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
Analysis of 5mC and 5hmC and associated transcription in trophoblast stem cells cultured and differentiated at 20% and 5% oxygen, integrated with previously published datasets
|
| |
|
| Overall design |
meDIP and hmeDIP was carried out to determine 5mC and 5hmC genomic distribution in tcontrol rophoblast stem cells and those lacking Tet1. hmeDIP and RNA-seq was also carried out in trophoblast stem cells cultured in 5% Oxygen to determine the effect of hypoxia on 5hmC and associated transcription
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| |
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| Contributor(s) |
Senner CE, Chrysanthou S, Burge S, Lin H, Branco M, Hemberger M |
| Citation(s) |
32442533 |
| Submission date |
Oct 17, 2019 |
| Last update date |
Dec 22, 2020 |
| Contact name |
Felix Krueger |
| E-mail(s) |
fkrueger@altoslabs.com
|
| Organization name |
The Babraham Institute
|
| Department |
Bioinformatics
|
| Street address |
Babraham Hall
|
| City |
Cambridge |
| ZIP/Postal code |
CB22 3AT |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (25)
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|
| Relations |
| BioProject |
PRJNA578148 |
| SRA |
SRP226100 |
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