|
| Status |
Public on Mar 31, 2020 |
| Title |
Analysis of NSL knockout brains |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In the current study, we analyse the function of MOF-NSL complex in neural cells. We find that the NSL complex regulates metabolic networks in the brain.
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| |
|
| Overall design |
Knockout of NSL complex members Mof, Kansl2 and Kansl3 was induced using the Nestin Cre. Neural cells, endothelial cells, pericytes and microglia were isolated by FACS. Gene expression analysed by RNA seq. and chromatin landscape via ChIP seq.
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| |
|
| Contributor(s) |
Sheikh B, Akhtar A, Reschler G |
| Citation(s) |
32541879 |
| |
| Submission date |
Oct 16, 2019 |
| Last update date |
Jul 09, 2020 |
| Contact name |
Asifa Akhtar |
| E-mail(s) |
akhtarlab_data@ie-freiburg.mpg.de
|
| Organization name |
Max Planck Institute of Immunobiology and Epigenetics
|
| Department |
Chromatin Regulation
|
| Lab |
Akhtar Lab
|
| Street address |
Stuebeweg 51
|
| City |
Freiburg |
| ZIP/Postal code |
79108 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (85)
|
|
| Relations |
| BioProject |
PRJNA577934 |
| SRA |
SRP225948 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE138981_Cre172_WT_H4K16ac.filtered.log2ratio.over_Cre172_WT_input.bw |
221.7 Mb |
(ftp)(http) |
BW |
| GSE138981_Cre172_WT_panH3.filtered.log2ratio.over_Cre172_WT_input.bw |
233.4 Mb |
(ftp)(http) |
BW |
| GSE138981_Cre173_WT_H4K16ac.filtered.log2ratio.over_Cre173_WT_input.bw |
193.8 Mb |
(ftp)(http) |
BW |
| GSE138981_Cre173_WT_panH3.filtered.log2ratio.over_Cre173_WT_input.bw |
210.0 Mb |
(ftp)(http) |
BW |
| GSE138981_FA-treated_DESeq_results.tsv.gz |
1.7 Mb |
(ftp)(http) |
TSV |
| GSE138981_FA-treated_gene_counts.tsv.gz |
403.4 Kb |
(ftp)(http) |
TSV |
| GSE138981_M621_KO_H4K16ac.filtered.log2ratio.over_M621_KO_input.bw |
164.7 Mb |
(ftp)(http) |
BW |
| GSE138981_M621_KO_panH3.filtered.log2ratio.over_M621_KO_input.bw |
213.9 Mb |
(ftp)(http) |
BW |
| GSE138981_M622_WT_H4K16ac.filtered.log2ratio.over_M622_WT_input.bw |
223.2 Mb |
(ftp)(http) |
BW |
| GSE138981_M622_WT_panH3.filtered.log2ratio.over_M622_WT_input.bw |
236.3 Mb |
(ftp)(http) |
BW |
| GSE138981_M662_KO_H4K16ac.filtered.log2ratio.over_M662_KO_input.bw |
159.5 Mb |
(ftp)(http) |
BW |
| GSE138981_M662_KO_panH3.filtered.log2ratio.over_M662_KO_input.bw |
200.1 Mb |
(ftp)(http) |
BW |
| GSE138981_M663_KO_H4K16ac.filtered.log2ratio.over_M663_KO_input.bw |
161.0 Mb |
(ftp)(http) |
BW |
| GSE138981_M663_KO_panH3.filtered.log2ratio.over_M663_KO_input.bw |
213.7 Mb |
(ftp)(http) |
BW |
| GSE138981_M666_WT_H4K16ac.filtered.log2ratio.over_M666_WT_input.bw |
250.2 Mb |
(ftp)(http) |
BW |
| GSE138981_M666_WT_panH3.filtered.log2ratio.over_M666_WT_input.bw |
264.8 Mb |
(ftp)(http) |
BW |
| GSE138981_endo_kansl2_vs_cre_DEseq_basic_DEresults.tsv.gz |
1.5 Mb |
(ftp)(http) |
TSV |
| GSE138981_endo_kansl2_vs_cre_DEseq_basic_counts_DESeq2.normalized.tsv.gz |
1011.6 Kb |
(ftp)(http) |
TSV |
| GSE138981_endo_kansl3_vs_cre_DEseq_basic_DEresults.tsv.gz |
1.5 Mb |
(ftp)(http) |
TSV |
| GSE138981_endo_kansl3_vs_cre_DEseq_basic_counts_DESeq2.normalized.tsv.gz |
1008.1 Kb |
(ftp)(http) |
TSV |
| GSE138981_endo_mof_vs_cre_DEseq_basic_DEresults.tsv.gz |
1.6 Mb |
(ftp)(http) |
TSV |
| GSE138981_endo_mof_vs_cre_DEseq_basic_counts_DESeq2.normalized.tsv.gz |
1.1 Mb |
(ftp)(http) |
TSV |
| GSE138981_microglia_mof_vs_cre_DEseq_basic_DEresults.tsv.gz |
1.4 Mb |
(ftp)(http) |
TSV |
| GSE138981_microglia_mof_vs_cre_DEseq_basic_counts_DESeq2.normalized.tsv.gz |
713.3 Kb |
(ftp)(http) |
TSV |
| GSE138981_neural_kansl2_vs_cre_DEseq_basic_DEresults.tsv.gz |
1.5 Mb |
(ftp)(http) |
TSV |
| GSE138981_neural_kansl2_vs_cre_DEseq_basic_counts_DESeq2.normalized.tsv.gz |
818.1 Kb |
(ftp)(http) |
TSV |
| GSE138981_neural_kansl3_vs_cre_DEseq_basic_DEresults.tsv.gz |
1.6 Mb |
(ftp)(http) |
TSV |
| GSE138981_neural_kansl3_vs_cre_DEseq_basic_counts_DESeq2.normalized.tsv.gz |
852.3 Kb |
(ftp)(http) |
TSV |
| GSE138981_neural_mof_vs_cre_DEseq_basic_DEresults.tsv.gz |
1.5 Mb |
(ftp)(http) |
TSV |
| GSE138981_neural_mof_vs_cre_DEseq_basic_counts_DESeq2.normalized.tsv.gz |
807.9 Kb |
(ftp)(http) |
TSV |
| GSE138981_pericytes_kansl2_vs_cre_DEseq_basic_DEresults.tsv.gz |
1.6 Mb |
(ftp)(http) |
TSV |
| GSE138981_pericytes_kansl2_vs_cre_DEseq_basic_counts_DESeq2.normalized.tsv.gz |
868.1 Kb |
(ftp)(http) |
TSV |
| GSE138981_pericytes_kansl3_vs_cre_DEseq_basic_DEresults.tsv.gz |
1.6 Mb |
(ftp)(http) |
TSV |
| GSE138981_pericytes_kansl3_vs_cre_DEseq_basic_counts_DESeq2.normalized.tsv.gz |
1002.6 Kb |
(ftp)(http) |
TSV |
| GSE138981_pericytes_mof_vs_cre_DEseq_basic_DEresults.tsv.gz |
1.6 Mb |
(ftp)(http) |
TSV |
| GSE138981_pericytes_mof_vs_cre_DEseq_basic_counts_DESeq2.normalized.tsv.gz |
850.9 Kb |
(ftp)(http) |
TSV |
| GSE138981_whole_brain_geneCounts.txt.gz |
549.3 Kb |
(ftp)(http) |
TXT |
| GSE138981_whole_brain_kansl2_differential_genes.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
| GSE138981_whole_brain_kansl3_differential_genes.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
| GSE138981_whole_brain_mof_differential_genes.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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