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Series GSE138971 Query DataSets for GSE138971
Status Public on Oct 17, 2019
Title Medroxyprogesterone acetate alters the vaginal microbiota and microenvironment in humans and increases susceptibility to HIV-1 in humanized mice
Organism Homo sapiens
Experiment type Other
Summary The hormonal contraceptive medroxyprogesterone acetate (MPA) is associated with increased risk of human immunodeficiency virus (HIV), via incompletely understood mechanisms. Increased diversity in the vaginal microbiota modulates genital inflammation and is associated with increased HIV-1 acquisition. However, the effect of MPA on diversity of the vaginal microbiota is relatively unknown. In a cohort of female Kenyan sex workers, negative for sexually transmitted infections (STIs), with Nugent scores <7 (N=58 of 370 screened), MPA correlated with significantly increased diversity of the vaginal microbiota as assessed by 16S rRNA gene sequencing. MPA was also significantly associated with decreased levels of estrogen in the plasma, and low vaginal glycogen and α-amylase, factors implicated in vaginal colonization by lactobacilli, bacteria that are believed to protect against STIs. In a humanized mouse model, MPA treatment was associated with low serum estrogen, low glycogen and enhanced HIV-1 susceptibility. The mechanism by which the MPA mediated changes in the vaginal microbiota may contribute to HIV-1 susceptibility in humans appears to be independent of inflammatory cytokines and/or activated T cells. Altogether, these results suggest MPA-induced hypo-estrogenism may alter key metabolic components that are necessary for vaginal colonization by certain bacterial species including lactobacilli, and allow for greater bacterial diversity in the vaginal microbiota.
 
Overall design Vaginal bacterial profiles of Kenyan sex workers were generated by 16S rRNA gene sequencing using the Illumina MiSeq platform.
 
Contributor(s) Wessels JM, Kaushic C
Citation(s) 31537512
Submission date Oct 16, 2019
Last update date Oct 17, 2019
Contact name Jocelyn M Wessels
Organization name McMaster University
Department Obstetrics and Gynecology
Street address 1280 Main Street West
City Hamilton
State/province Ontario
ZIP/Postal code L8S 4K1
Country Canada
 
Platforms (1)
GPL15520 Illumina MiSeq (Homo sapiens)
Samples (58)
GSM4125392 CK97 - OCP
GSM4125393 CK98 - NH
GSM4125394 CK99 - DMPA
Relations
BioProject PRJNA577911
SRA SRP225929

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138971_otu_table_rdp-training_gg2011_L6.xlsx 155.7 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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