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| Status |
Public on Feb 27, 2020 |
| Title |
ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesin-STAG1 from WAPL |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The human genome folds to create thousands of loops connecting sites that are bound by the insulator protein CTCF and the ring-shaped cohesin complex. It is thought that most of these loops emerge through a process whereby cohesin extrudes chromatin, forming an initially small loop that grows larger and larger until the loop’s expansion is arrested by CTCF. Cohesin rings comprise four proteins: SMC1, SMC3, SCC1, and, in higher eukaryotes, either STAG1 or STAG2. We explore differential roles of especially STAG1, STAG2 and ESCO1 proteins in chromatin organization.
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| Overall design |
We combine Hi-C and ChIP-Seq data with FRAP and physical simulations to dissect the roles of these two forms of cohesin - STAG1 and STAG2 - in G1 phase.
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| Contributor(s) |
Stocsits RR, Wutz G, Tang W, Schoenfelder S, Ivanov M, Varnai C, St.Hilaire B |
| Citation(s) |
32065581 |
| |
| Submission date |
Oct 03, 2019 |
| Last update date |
Mar 18, 2020 |
| Contact name |
Roman R Stocsits |
| E-mail(s) |
roman.stocsits@imp.ac.at
|
| Organization name |
IMP - Research Institute of Molecular Pathology
|
| Lab |
Jan-Michael Peters' Lab
|
| Street address |
Campus-Vienna-Biocenter 1
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (25)
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| GSM4106788 |
Hi-C: G1 sync. Control - HindIII - 2 reps. |
| GSM4106789 |
Hi-C: HeLa G1 High Resolution control - MboI |
| GSM4106790 |
Hi-C: STAG1 depleted high resolution |
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| Relations |
| BioProject |
PRJNA575745 |
| SRA |
SRP224133 |
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