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Series GSE138220 Query DataSets for GSE138220
Status Public on Apr 27, 2020
Title A Tudor-domain containing protein, SIMR-1, mediates silencing of piRNA target mRNAs by the Mutator Complex
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary piRNAs play a critical role in the regulation of transposons and other germline genes. In Caenorhabditis elegans, silencing of piRNA target genes is mediated by the Mutator complex, which synthesizes high levels of siRNAs through the activity of an RNA-dependent RNA polymerase. However, how mRNAs recognized by the piRNA pathway are handed off to the Mutator pathway is unclear. Here, we identify the Tudor domain-containing protein, SIMR-1, as a key mediator of this handoff. Interestingly, SIMR-1 also localizes to distinct subcellular foci adjacent to P granules and Mutator foci, two phase-separated condensates that are the sites of piRNA-dependent mRNA recognition and Mutator complex-dependent siRNA amplification, respectively. Thus, our data suggests a role for multiple perinuclear condensates in organizing the piRNA pathway and promoting silencing by the Mutator complex.
Overall design 10 small-RNA seq libraries in duplicate, 14 single replicate small-RNA seq libraries, and 10 mRNA-seq libraries in triplicate were generated and analyzed.
Web link
Contributor(s) Phillips C, Manage K, Rogers A, Uebel C, Arca K
Citation(s) 32338603
Submission date Sep 30, 2019
Last update date Apr 29, 2020
Contact name Alicia Kathryn Rogers
Phone 8172722873
Organization name University of Texas at Arlington
Department Department of Biology
Street address 1225 W Mitchell Street
City Arlington
State/province Texas
ZIP/Postal code 76019
Country USA
Platforms (1)
GPL19757 Illumina NextSeq 500 (Caenorhabditis elegans)
Samples (64)
GSM4102118 N2 gen0 rep 1
GSM4102119 N2 gen0 rep 2
GSM4102120 N2 gen1 rep 1
BioProject PRJNA575051
SRA SRP223779

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Supplementary file Size Download File type/resource
GSE138220_RAW.tar 52.1 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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