|
| Status |
Public on Dec 20, 2019 |
| Title |
Glioma-derived miRNA-containing extracellular vesicles induce angiogenesis by reprogramming brain endothelial cells (longRNA-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Glioblastoma multiforme (GBM) is characterized by the close relationship of glioma stem cells (GSC) with aberrant vascularization. It has been established that GSC-derived extracellular vesicles (GSC-EVs) and their cargoes are proangiogenic in vitro. To elucidate gene regulatory mechanisms of neovascularization both in vitro and in vivo, we performed RNA-seq and DNA methylation profiling of the response of human brain endothelial cells to GSC-EVs as well as histoepigenetic analysis of GBM molecular profiles in the TCGA collection. The gene regulatory responses showed a footprint of post-transcriptional gene silencing by EV-derived miRNAs. Remarkably, EVs and normal vascular growth factors stimulated highly distinct gene regulatory responses, both converging on angiogenesis, providing exciting new insight into targetable angiogenic signaling in GBM.
|
| |
|
| Overall design |
In this study, DNA methylation and gene expression profiling by long-RNA-seq and small-RNA-seq were performed on cultured human brain microvascular endothelial cells to investigate expression patterns of angiogenesis.
|
| |
|
| Contributor(s) |
Lucero RD, Zappulli V, Sammarco A, Murillo OD, Cheah PS, Srinivasan S, Tai E, Ting DT, Wei Z, Roth ME, Laurent LC, Krichevsky AM, Breakefield XO, Milosavljevic A |
| Citation(s) |
32075753 |
| |
| Submission date |
Sep 27, 2019 |
| Last update date |
Mar 20, 2020 |
| Contact name |
Aleksandar Milosavljevic |
| E-mail(s) |
amilosav@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Genetics and Genomics
|
| Lab |
Bioinformatics Research Laboratory
|
| Street address |
1 Baylor Plaza
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE138115 |
Glioma-derived miRNA-containing extracellular vesicles induce angiogenesis by reprogramming brain endothelial cells |
|
| Relations |
| BioProject |
PRJNA574644 |
| SRA |
SRP223530 |
Less...
More...