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| Status |
Public on Apr 28, 2020 |
| Title |
METTL5, an 18S rRNA-specific m6A methyltransferase, modulates expression of stress response genes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Input mRNAs and ribosome protected fragments (RPFs) from wildtype and Mettl5 knockout (KO) B16 cells were analyzed by TruSeq Ribo Profile (Illumina) library preparation and high throughput sequencing
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| Overall design |
Mouse melanoma B16 cells were grown in standard conditions (DMEM + 10% FBS + Pen/Strep (100U / mL)). Input mRNAs and RPFs were isolated, ribosomal RNAs were depleted and cDNA libraries were prepared according to the TruSeq Ribo Profile pipeline (Illumina)
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| Web link |
https://www.biorxiv.org/content/10.1101/2020.04.27.064162v1
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| |
|
| Contributor(s) |
Chen H, Liu Q, Shi Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 27, 2019 |
| Last update date |
Apr 28, 2020 |
| Contact name |
Yang Shi |
| Organization name |
BOSTON CHILDREN'S HOSPITAL
|
| Department |
NEWBORN MEDICINE
|
| Street address |
320 LONGWOOD AVE
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA574637 |
| SRA |
SRP223527 |
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