|
| Status |
Public on Jan 31, 2011 |
| Title |
Identification and validation of gene expression changes upon removal of Dicer in the murine limb mesenchyme |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Wild type and Dicer null embryonic mouse limbs were analaysed using Affymetrix arrays to identify gene expression changes. Genes that were up-regulated in Dicer-null limbs were canidates for being miRNA targets.
Potential miRNA target genes were validated using qRTPCR.
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| |
|
| Overall design |
Mice carrying a heterozygous Dicer floxed allele and the prxcre driver allele were crossed to homozygous Dicer Floxed mice. Embryos were harvested from pregent moms and genotyped to determine heterozygous, Wild type and mutant limb buds. These limb buds were used to prepare RNA for array analysis.
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| |
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| Contributor(s) |
Maatouk DM, Gaskins III RB, O'Rourke JR, Swanson MS, Lopez MC, McManus MT, Baker HV, Harfe BD |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 03, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
M Cecilia Lopez |
| E-mail(s) |
mclopez@ufl.edu
|
| Phone |
352 273-8133
|
| Organization name |
University of Florida
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Microarray lab 103
|
| Street address |
1376 Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610-3610 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA110575 |
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