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Series GSE138030 Query DataSets for GSE138030
Status Public on Sep 25, 2020
Title Transcriptome analysis of HeLa cells knocked down for EXT1 or FBXW7 gene expression
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Exostosin-1 (EXT1) is an ER-resident glycosyltransferase known to polymerize heparan sulfate chain by sequential addition of glucuronic acid and N-acetylglucosamine molecules. This reaction results in heavily glycosylated proteoglycans (PG) at the plasma membrane, which form cell-type specific complexes and play vital roles in cell-cell and cell-pathogens interactions, cell growth, plasticity and proliferation in the local tissue environment. We found that EXT1 interact with a number of intracellular proteins including FBXW7 and hypothesized that both proteins cooperate in targeted protein degradation.
Overall design Examination of transcriptomic differences following knockdown of EXT1 or FBXW7.
Contributor(s) Daakour S, Hajingabo L, Kerselidou D, Twizere J
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Submission date Sep 25, 2019
Last update date Sep 25, 2020
Contact name Jean-Claude Twizere
Phone +3243669981
Organization name GIGA Institute
Department Molecular Biology of Diseases
Lab Viral Interactomes Lab
Street address CHU - B34 Quartier Hôpital Avenue de l'Hôpital,11
City Liège
State/province Liège
ZIP/Postal code 4000
Country Belgium
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (4)
GSM4097043 HeLa cells, siCTRL
GSM4097044 HeLa cells, siEXT1
GSM4097045 HeLa cells, siFBXW7
BioProject PRJNA574199
SRA SRP223302

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138030_Table_S5A_deregulated_genes_siExt1.xlsx 50.9 Kb (ftp)(http) XLSX
GSE138030_Table_S5B_deregulated_genes_siFbxw7.xlsx 43.4 Kb (ftp)(http) XLSX
GSE138030_Table_S5C_intersection_deregulated_genes_siExt1_siFbxw7.xlsx 34.8 Kb (ftp)(http) XLSX
GSE138030_Table_S5D_genes_expression_siExt1.xlsx 530.8 Kb (ftp)(http) XLSX
GSE138030_Table_S5E_genes_upregulated_siExt1.xlsx 56.9 Kb (ftp)(http) XLSX
GSE138030_Table_S5F_genes_downregulated_siExt1.xlsx 36.8 Kb (ftp)(http) XLSX
GSE138030_Table_S5G_genes_expression_siFbxw7.xlsx 621.3 Kb (ftp)(http) XLSX
GSE138030_Table_S5H_genes_upregulated_siFbxw7.xlsx 41.2 Kb (ftp)(http) XLSX
GSE138030_Table_S5I_genes_downregulated_siFbxw7.xlsx 43.2 Kb (ftp)(http) XLSX
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