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| Status |
Public on Sep 15, 2020 |
| Title |
Cortical RORb is required for layer 4 transcriptional identity and barrel integrity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
RNA-seq and ATAC-seq from layer 4 cortical neurons expressing RORb-gfp or GFP in an RORb KO.
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| |
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| Overall design |
RNA-seq and ATAC-seq from FACS isolated neruons. RNA-seq was carried out on ~1000 L4 S1 neurons from mice of different ages (P2, P7, and P30). ATAC-seq was carred out on ~40,000 L4 neurons from P28-P30 mice. All samples were collected from a pool of cells taken from at least 1 male and 1 female.
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| |
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| Contributor(s) |
Nelson S, Clark E, Myers E, Schulmann A |
| Citation(s) |
32851975 |
| |
| Submission date |
Sep 25, 2019 |
| Last update date |
Sep 21, 2020 |
| Contact name |
Erin Amelia Clark |
| E-mail(s) |
erin.a.clark@gmail.com
|
| Organization name |
Brandeis University
|
| Department |
Biology
|
| Lab |
Sacha Nelson
|
| Street address |
415 South St
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02453 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA574131 |
| SRA |
SRP223255 |
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