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| Status |
Public on Dec 20, 2019 |
| Title |
Glioma-derived miRNA-containing extracellular vesicles induce angiogenesis by reprogramming brain endothelial cells (DNA methylation) |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Glioblastoma multiforme (GBM) is characterized by the close relationship of glioma stem cells (GSC) with aberrant vascularization. It has been established that GSC-derived extracellular vesicles (GSC-EVs) and their cargoes are proangiogenic in vitro. To elucidate gene regulatory mechanisms of neovascularization both in vitro and in vivo, we performed RNA-seq and DNA methylation profiling of the response of human brain endothelial cells to GSC-EVs as well as histoepigenetic analysis of GBM molecular profiles in the TCGA collection. The gene regulatory responses showed a footprint of post-transcriptional gene silencing by EV-derived miRNAs. Remarkably, EVs and normal vascular growth factors stimulated highly distinct gene regulatory responses, both converging on angiogenesis, providing exciting new insight into targetable angiogenic signaling in GBM.
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| Overall design |
In this study, DNA methylation and gene expression profiling by long-RNA-seq and small-RNA-seq were performed on cultured human brain microvascular endothelial cells to investigate expression patterns of angiogenesis.
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| Contributor(s) |
Lucero RD, Zappulli V, Breakefield XO, Milosavljevic A |
| Citation(s) |
32075753 |
| |
| Submission date |
Sep 23, 2019 |
| Last update date |
Mar 20, 2020 |
| Contact name |
Aleksandar Milosavljevic |
| E-mail(s) |
amilosav@bcm.edu
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| Organization name |
Baylor College of Medicine
|
| Department |
Genetics and Genomics
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| Lab |
Bioinformatics Research Laboratory
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| Street address |
1 Baylor Plaza
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| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (9)
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| GSM4089158 |
genomic DNA from HBMVECs in EBM plus angiogenic growth factors_B1 |
| GSM4089159 |
genomic DNA from HBMVECs in EBM plus angiogenic growth factors_B2 |
| GSM4089160 |
genomic DNA from HBMVECs in EBM plus angiogenic growth factors_B3 |
| GSM4089161 |
genomic DNA from HBMVECs in EBM plus EVs isolated from GBM8 neurosphere culture_C1 |
| GSM4089162 |
genomic DNA from HBMVECs in EBM plus EVs isolated from GBM8 neurosphere culture_C2 |
| GSM4089163 |
genomic DNA from HBMVECs in EBM plus EVs isolated from GBM8 neurosphere culture_C3 |
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| This SubSeries is part of SuperSeries: |
| GSE138115 |
Glioma-derived miRNA-containing extracellular vesicles induce angiogenesis by reprogramming brain endothelial cells |
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| Relations |
| BioProject |
PRJNA573609 |
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