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| Status |
Public on Oct 05, 2019 |
| Title |
Heat Shock Factor 1 (HSF1) acquires transcriptional competence under 17b-estradiol in ERa-positive breast cancer cells [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Heat Shock Transcription Factor 1 (HSF1) is a well-known regulator of gene expression during acute environmental stress that enables the cells to survive. Its high level in estrogen receptor-positive breast cancer patients correlated with a worse prognosis. Here, we demonstrated that 17β-estradiol (E2) as well as bisphenol A (BPA) and propyl pyrazole triol (PPT, ERα agonist) led to HSF1 phosphorylation on S326 in ERα positive mammary breast cancer cells, but not in ERα-negative ones. We showed that ERK1/2 signaling was involved in this process and down-regulation of ERα expression abrogated it. E2activated HSF1 was transcriptionally potent. Chip-Seq and RNA-Seq analyses revealed that it could modulate the expression of several genes known to be essential for breast cancer cells growth and/or ERα action, i.e. HSPB8, LHX4, PRKCE, WWC1, and GREB1. Our findings indicate that a positive feedback loop between ERα and HSF1 signaling may exist which support the growth of estrogen-dependent tumors.
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| Overall design |
We sequenced mRNA from wild type MCF7 cells. Cells were untreated (control), stimulated using estrogen (17beta-estradiol) for indicated time or subjected to hyperthermia with subsequent recovery. Three biological replicates were performer, pooled and sequenced.
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| Contributor(s) |
Vydra N, Widlak W, Stokowy T |
| Citation(s) |
31614463 |
| |
| Submission date |
Sep 17, 2019 |
| Last update date |
Nov 04, 2019 |
| Contact name |
Tomasz Stokowy |
| E-mail(s) |
tomasz.stokowy@uib.no
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| Organization name |
University of Bergen
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| Department |
IT Division
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| Lab |
Scientific Computing
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| Street address |
Nygardsgaten 5
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| City |
Bergen |
| ZIP/Postal code |
5020 |
| Country |
Norway |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE137560 |
Heat Shock Factor 1 (HSF1) acquires transcriptional competence under 17b-estradiol in ERa-positive breast cancer cells |
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| Relations |
| BioProject |
PRJNA565925 |
| SRA |
SRP221987 |
