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Series GSE137439 Query DataSets for GSE137439
Status Public on Sep 25, 2019
Title Glycine Cleavage System Determines the Fate of Pluripotent Stem Cells via the Regulation of Senescence and Epigenetic Modifications
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigate whether embryonic stem cells exhibit a metabolic state different from that of somatic cells, we performed RNA-seq in mouse embryonic fibroblasts (MEFs), induced pluripotent stem cells (iPSCs) and mouse embryonic stem cells (mESCs). To further investigate the effect of Gldc in mESCs, we performed RNA-seq in V6.5 cells with Gldc knockdown.
Overall design RNA-seq for MEF,IPS and V6.5 cells was performed to analyze the gene expression of these lines. mouse ES cell were transfected with Gldc shRNA or non-targeting control shRNA followed by RNA extraction and sequenced on HiSeq X Ten (Mus musculus).
Contributor(s) Tian S, Feng J, Shen S, Zhang H, Zhong X, Gao P
Citation(s) 31562192
Submission date Sep 13, 2019
Last update date Oct 08, 2019
Contact name junru feng
Phone +860051 63607033
Organization name USTC
Street address huangshan road #443
City Hefei
ZIP/Postal code 230026
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (5)
GSM4079345 MEF
GSM4079346 IPS
GSM4079347 V6.5
BioProject PRJNA565440
SRA SRP221611

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137439_IPS_V6.5_MEF.ballgown.txt.gz 365.9 Kb (ftp)(http) TXT
GSE137439_V6.5_shGLDC.ballgown.txt.gz 256.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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