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| Status |
Public on Sep 14, 2019 |
| Title |
Loss-of-function of the long non-coding RNA A830019P07Rik in mice does not affect insulin expression and secretion |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Long non-coding RNAs (lncRNAs) contribute to diverse cellular functions and the dysregulation of their expression or function can contribute to diseases, including diabetes. The contributions of lncRNAs to β-cell development, function and survival has been extensively studied in vitro. However, very little is currently known on the in vivo roles of lncRNAs in the regulation of glucose and insulin homeostasis. Here we investigated the impact of loss-of-function in mice of the lncRNA A830019P07Rik, hereafter P07Rik, which was previously reported to be associated with reduced plasma insulin levels. Compared with wild-type littermates, male and female P07Rik mutant mice did not show any defect in glycaemia and plasma insulin levels in both fed and fasted state. Furthermore, P07Rik mutant mice displayed similar glucose and insulin levels in response to an intra-peritoneal glucose tolerance test. Ex vivo, islets from mutant P07Rik released similar amount of insulin in response to increased glucose concentration as wildtype littermates. In contrast with previous reports, our characterization of P07Rik mouse mutants revealed that loss of function of this lncRNA does not affect glucose and insulin homeostasis in mice.
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| Overall design |
RNAseq of mouse islet in P07Rik KO and WT mice
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| Contributor(s) |
Guay C, Abdulkarim B, Tan JY, Dubuis G, Rütti S, Widmann C, Regazzi R, Marques AC |
| Citation(s) |
32286361 |
| Submission date |
Sep 13, 2019 |
| Last update date |
Apr 27, 2020 |
| Contact name |
Jennifer Yihong Tan |
| E-mail(s) |
jennifer.tan@unil.ch
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| Phone |
+4121 692 5472
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| Organization name |
University of Lausanne
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| Department |
Department of Computational Biology
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| Lab |
Ana Marques
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| Street address |
Rue du Bugnon 27
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| City |
Lausanne |
| ZIP/Postal code |
1005 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA565363 |
| SRA |
SRP221554 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE137389_P007Rik_count_table.txt.gz |
141.5 Kb |
(ftp)(http) |
TXT |
| GSE137389_P007Rik_paired_DEG_table.txt.gz |
707.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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