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Series GSE137389 Query DataSets for GSE137389
Status Public on Sep 14, 2019
Title Loss-of-function of the long non-coding RNA A830019P07Rik in mice does not affect insulin expression and secretion
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Long non-coding RNAs (lncRNAs) contribute to diverse cellular functions and the dysregulation of their expression or function can contribute to diseases, including diabetes. The contributions of lncRNAs to β-cell development, function and survival has been extensively studied in vitro. However, very little is currently known on the in vivo roles of lncRNAs in the regulation of glucose and insulin homeostasis. Here we investigated the impact of loss-of-function in mice of the lncRNA A830019P07Rik, hereafter P07Rik, which was previously reported to be associated with reduced plasma insulin levels. Compared with wild-type littermates, male and female P07Rik mutant mice did not show any defect in glycaemia and plasma insulin levels in both fed and fasted state. Furthermore, P07Rik mutant mice displayed similar glucose and insulin levels in response to an intra-peritoneal glucose tolerance test. Ex vivo, islets from mutant P07Rik released similar amount of insulin in response to increased glucose concentration as wildtype littermates. In contrast with previous reports, our characterization of P07Rik mouse mutants revealed that loss of function of this lncRNA does not affect glucose and insulin homeostasis in mice.
Overall design RNAseq of mouse islet in P07Rik KO and WT mice
Contributor(s) Guay C, Abdulkarim B, Tan JY, Dubuis G, Rütti S, Widmann C, Regazzi R, Marques AC
Citation(s) 32286361
Submission date Sep 13, 2019
Last update date Apr 27, 2020
Contact name Jennifer Yihong Tan
Phone +4121 692 5472
Organization name University of Lausanne
Department Department of Computational Biology
Lab Ana Marques
Street address Rue du Bugnon 27
City Lausanne
ZIP/Postal code 1005
Country Switzerland
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM4078103 KO2775
GSM4078104 KO2776
GSM4078105 WT2772
BioProject PRJNA565363
SRA SRP221554

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Supplementary file Size Download File type/resource
GSE137389_P007Rik_count_table.txt.gz 141.5 Kb (ftp)(http) TXT
GSE137389_P007Rik_paired_DEG_table.txt.gz 707.3 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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