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| Status |
Public on Sep 01, 2020 |
| Title |
Transcriptional control of influenza-specific CD4+ tissue-resident memory T cell generation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Tissue-resident memory T cells (TRM) are a non-circulating subset of memory that are maintained at sites of pathogen entry and mediate optimal protection against reinfection. Lung TRM can be generated in response to respiratory infection or vaccination, however, the molecular pathways involved in CD4+TRM establishment have not been defined. Here, we performed transcriptional profiling of influenza-specific lung CD4+TRM following influenza infection to identify pathways implicated in CD4+TRM generation and homeostasis. Lung CD4+TRM displayed a unique transcriptional profile distinct from spleen memory, including up-regulation of a gene network induced by the transcription factor IRF4, a known regulator of effector T cell differentiation.
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| |
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| Overall design |
Transcriptional profiling by RNA-Seq of lung-resident and spleen influenza-specific mouse CD4+ T cells. Project includes four and five biological replicates of each sample type, respectively.
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| Contributor(s) |
Cvetkovski F, Snyder ME, Farber DL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 13, 2019 |
| Last update date |
Sep 01, 2020 |
| Contact name |
Donna L Farber |
| E-mail(s) |
df2396@columbia.edu
|
| Organization name |
Columbia University
|
| Department |
Surgery
|
| Street address |
650 West 168th Street, BB1701
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA565358 |
| SRA |
SRP221552 |
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