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Series GSE137299 Query DataSets for GSE137299
Status Public on Apr 09, 2020
Title Characterization of cochlear development at the single cell level
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The organ of Corti, located in the floor of the scala media of the cochlea, acts as the primary sensory transducer of sound in mammals.  This remarkable structure comprises a highly diverse cellular mosaic that includes two unique types of mechanosensory hair cells and an undefined number of associated supporting cell types.  All of these cells are believed to arise from a developmental equivalence group referred to as the prosensory domain that is similarly thought to arise from a proneurosensory population that develops in the anterior-ventral region of the otocyst.  The results of both classical embryologic manipulations and modern molecular genetic experiments suggest that otocyst precursor cells proceed through several rounds of lineage restriction that progressively specify subsets of cells as prosensory cells and ultimately as either hair cells or supporting cells.  However, the relatively small sizes of the otocyst and inner ear have hindered efforts to determine the full diversity of cell types within the mature cochlea and to identify the transitional cell types that exist during development.  The recent development of droplet-based methods for isolation and subsequent transcriptional profiling of individual cells provides a potential method to address both of these challenges.  Therefore, we dissected, dissociated and then captured over 27,000 epithelial cells from the developing cochlear duct at specific time points between E14 and P7.
Overall design We characterized the transcriptomic profiles of cochlear epithelium celltypes of E14, E16, P1, P7 wild type (WT) mice at a single cell resolution using 10x Chromium  and Illumina sequencing platforms. Data contain over 27,000 single cells.
Contributor(s) Kelly MC, Kolla L, Kelley MW, Morell RJ
Citation(s) 32404924
Submission date Sep 11, 2019
Last update date Nov 21, 2022
Contact name Robert Morell
Organization name National Institute on Deafness and Other Communication Disorders
Street address 35A Convent Drive, Room 1F-103
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (11)
GSM4074928 E14se_1
GSM4074929 E14se_2
GSM4074930 E16se_1
BioProject PRJNA565059
SRA SRP221409

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Supplementary file Size Download File type/resource
GSE137299_E14_Normalized_Counts.txt.gz 91.0 Mb (ftp)(http) TXT
GSE137299_E16_Normalized_Counts.txt.gz 96.0 Mb (ftp)(http) TXT
GSE137299_P1_Normalized_Counts.txt.gz 145.3 Mb (ftp)(http) TXT
GSE137299_P7_Normalized_Counts.txt.gz 55.0 Mb (ftp)(http) TXT
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