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| Status |
Public on Sep 12, 2019 |
| Title |
Single-nucleus chromatin accessibility maps of IDH mutant gliomas point to non-coding RNAs as a major influence on heterogeneity |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We generated chromatin accessibility maps from 5 tumors, 336 individual nuclei, using the Fluidigm HT IFC microfluidics platform (snATAC-Seq). We were able to identify variable promoter accessibility of non-coding RNAs in LGGs.
Our results point to increased CYTOR promoter accessibility in IDH-intact samples. We obtained two distinct clusters, primarily driven by histology, with a more constrained IDH-codel/oligodendroglioma cluster.
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| |
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| Overall design |
Examination of open chromatin accessibility in 336 nuclei from 5 low grade tumors
>>>Submitter states that raw data are unavailable due to patient privacy concerns<<<
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| Contributor(s) |
Al-Ali R, Turcan S |
| Citation(s) |
31806013 |
| |
| Submission date |
Sep 11, 2019 |
| Last update date |
Dec 12, 2019 |
| Contact name |
Ruslan Al-Ali |
| Organization name |
University Hospital Heidelberg
|
| Lab |
Sevin Turcan
|
| Street address |
INF 400
|
| City |
Heidelberg |
| State/province |
Baden Wurttemberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA564978 |
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