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Series GSE137196 Query DataSets for GSE137196
Status Public on Dec 10, 2019
Title Genome-wide mapping of ligand-dependent ERα binding sites in the ERα AF-2 mutant mouse uterus
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Estrogen receptor alpha (ERα) is a ligand dependent transcription regulator, which contains two transactivation functional domains, AF-1 and AF-2. These activities are regulated differently by the ligands. Specifically, the selective estrogen receptor modulators (SERMs) regulate AF-1 rather than AF-2. It is important to know whether AF-1/AF-2 predominantly regulated genes exist in the tissues for a better understanding of the SERMs functionality. We sought out AF-1 dependent estrogenic genes by using the AF-2 mutated knock-in (KI) mouse model, AF2ERKI. AF2ER is an estrogen-insensitive AF-2 disrupted ERα mutant mouse but unique to this model, AF-1 can be activated by the estrogen-antagonists, such as fulvestrant (ICI) and 4-hydroxytamoxifen (Tam) in vitro and in vivo. The information of genome-wide ICI or Tam dependent AF-2 mutated ERα binding sites could explain the mechanism of the selective estrogenic action of SERMs through AF-1 dependent gene regulation.
Overall design Ovariectomized (OVX) mice were treated with ICI, Tam or vehicle for 1h. Uterus was collected from the six mice in each treatment then put together for a sample.Each dataset was obtained from a single pool of uterine tissue (n = 6 mice)
Contributor(s) Arao Y, Hamilton KJ, Grimm SA, Korach KS
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Submission date Sep 10, 2019
Last update date Dec 12, 2019
Contact name Yukitomo Arao
Phone 9842874322
Organization name NIEHS
Street address 111 TW Alexandr Dr.
City Durham
State/province NC
ZIP/Postal code 27009
Country USA
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM4072808 Af2KI_E1h_ICI_ER
GSM4072809 Af2KI_E1h_Tam_ER
GSM4072810 Af2KI_E1h_Veh_ER
BioProject PRJNA564792
SRA SRP221248

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Supplementary file Size Download File type/resource
GSE137196_RAW.tar 1.5 Gb (http)(custom) TAR (of BED, BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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