|
| Status |
Public on Oct 16, 2019 |
| Title |
Single Cell RNAseq profiles of thymic ETP populations |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The single cell profiles of sorted ETP populations with cell hashing antibody barcoding from mouse thymus
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| |
|
| Overall design |
Examination of single cell expression profile of sorted populations identified from previous pseudotime analysis. Experiment was performed using droplet-based 3′ end RNA expression profiling using Chromium Single Cell 3′ Reagent Kits v3 according to manufacturer’s protocol (10x Genomics), with the FACS sorted individual sub-populations of ETPs tagged with Totalseq-A hashtags (Biolegend). The cDNA library was sequenced on Illumina Novaseq for 400 million reads, and tag library on HiSeq4000 for 20 million reads, both in pair-end mode following manufacturer's instructions.
Please note that meta.csv.gz contains assigned sample names of individual cells after demultiplexing
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| |
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| Contributor(s) |
Zhou W, Yui MA, Williams BA, Yun J, Wold BJ, Cai L, Rothenberg EV |
| Citation(s) |
31629685 |
| |
| Submission date |
Sep 09, 2019 |
| Last update date |
Jan 15, 2020 |
| Contact name |
Wen Zhou |
| E-mail(s) |
wenzhou.caltech@gmail.com
|
| Phone |
6263954915
|
| Organization name |
California Institute of Technology
|
| Department |
BBE
|
| Lab |
Ellen Rothenberg
|
| Street address |
1200 E California Blvd, Mail Code 250-86
|
| City |
Pasadena |
| State/province |
California |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (2) |
| GSM4072329 |
Sorted 4 ETP subpopulations and ETP-DN2 controls |
| GSM4072330 |
Antibody sample tag library for ETP subpop |
|
| Relations |
| BioProject |
PRJNA564688 |
| SRA |
SRP221183 |
