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Series GSE13711 Query DataSets for GSE13711
Status Public on Nov 25, 2009
Title Comparative transcriptome and phenotype analysis of acid-stressed Bacillus cereus strain ATCC 14579
Platform organism Bacillus cereus ATCC 14579
Sample organism Bacillus cereus
Experiment type Expression profiling by array
Summary The food-borne human pathogen Bacillus cereus is found in environments that often have a low pH, such as food and soil. The physiological response upon exposure to several levels of acidity were investigated of B. cereus model strain ATCC 14579, to elucidate the response of B. cereus to acid stress. pH 5.4, pH 5.0, pH 4.8 and pH 4.5 were selected to conduct microarray analyses, based on the differences in physiological response upon exposure to the acid conditions. The transcriptome data revealed response specific profiles. Showing mechanisms induced upon all the different acid down-shocks, such as nitrate reductase and energy production genes, and several genes specifically expressed differentially in mild or lethal levels of acidity, such as F1F0-ATPase and cydAB. Furthermore, mechanisms involved in oxidative stress response were found highly up-regulated in response to both mild and lethal acid stress. The induction of oxidative stress related genes may be a response to the formation of reactive oxygen species by a perturbation of the electron transport chain. Therefore, the formation of hydroxyl radicals and/ or peroxynitrite was monitored upon exposure to the different levels of acidity with a fluorescent probe in a flow cytometer. The formation of these oxidative compounds was shown to be specific for lethal pHs and a model to relate radical formation with the observed transcriptome profiles was proposed.
 
Overall design Per acid down-shock three exposure times (i.e., 10, 30 and 60 min) were each compared with non-exposed cells (i.e., t0). In total 4 different acid down-shocks were applied, pH 5.4, pH 5.0, pH 4.8 and pH 4.5. The experiments were performed in duplicate and the duplicate samples were hybridised with a dye-swap.
 
Contributor(s) Mols M, van Kranenburg R, van Melis C, Moezelaar R, Abee T
Citation(s) 20074238
Submission date Nov 22, 2008
Last update date Mar 20, 2012
Contact name Maarten Mols
Organization name Wageningen University
Department ATV
Lab Food Microbiology
Street address Bomenweg 2
City Wageningen
ZIP/Postal code 6703 HD
Country Netherlands
 
Platforms (1)
GPL7680 Bacillus cereus Bc14579 - 2nd design (2x11K)
Samples (24)
GSM344294 Bc14579 pH5.4 t0_t10
GSM344296 Bc14579 pH5.4 t0_t30
GSM344729 Bc14579 pH5.4 t0_t60
This SubSeries is part of SuperSeries:
GSE13773 Comparative transcriptome and phenotype analysis of acid-stressed Bacillus cereus strains
Relations
BioProject PRJNA114641

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Supplementary data files not provided
Raw data provided as supplementary file
Processed data included within Sample table
Raw data included within Sample table

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