|
| Status |
Public on Dec 11, 2019 |
| Title |
CRISPR screening reveals targets to reprogram long-lived effector CD8+ T cells for cancer therapy [scRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
CD8+ T cells can be reprogrammed for better persistence and robust effector function in TME. By performing an in vivo pooled CRISPR-Cas9 mutagenesis screening of metabolism-associated factors, we identify Regnase-1 as a major negative regulator of antitumor responses, whose deficiency results in drastically increased CD8+ T cell accumulation in tumors
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| |
|
| Overall design |
We used scRNA seq to compare transcriptional profiles of Regnase1-null and WT CD8+ T cells population from tumors.
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| |
|
| Contributor(s) |
Wei J, Long L, Dhungana Y, Chi H |
| Citation(s) |
31827283 |
| Submission date |
Sep 06, 2019 |
| Last update date |
Dec 20, 2019 |
| Contact name |
Hongbo Chi |
| E-mail(s) |
hongbo.chi@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Immunology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE126072 |
CRISPR screening reveals targets to reprogram long-lived effector CD8+ T cells for cancer therapy. |
|
| Relations |
| BioProject |
PRJNA564283 |
| SRA |
SRP220659 |
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