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| Status |
Public on Sep 07, 2019 |
| Title |
Gene expression profiles in rat alveolar macrophages exposure to multi-walled carbon nanotubes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
To evaluate the biological effects at the transcriptional level of manufactured nanomaterials, we performed whole genome microarrays of rat alveolar macrophages (NR8383) exposed to multi-walled carbon nanotubes (MWCNTs) with different physicochemical properties.
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| Overall design |
Rat alveolar macrophages (NR8383) were exposed to 100 µg/mL of rigid needle-like shaped MWCNTs (MW1 and MW2), or bent-shaped MWCNTs (MW3 and MW4), and 50 µg/mL of straight and thin-shaped MWCNTs (MW5 and MW6) for 24 hours, respectively. The experiments were performed using control for each experiment (n=4). Gene expression profiles in rat alveolar macrophages exposure to submicron carbon fibers Cyanine-3-labeled cRNA prepared from cells was hybridized to a Whole Rat Genome Microarray. The scanned images of hybridized microarray slides were analyzed numerically to evaluate the biological effects at the transcriptional level of MWCNTs with different physicochemical properties on NR8383.
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| Web link |
https://doi.org/10.1080/15376516.2020.1761920
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| Contributor(s) |
Fujita K, Obara S |
| Citation(s) |
32345130 |
| |
| Submission date |
Sep 06, 2019 |
| Last update date |
Dec 10, 2024 |
| Contact name |
Katsuhide Fujita |
| E-mail(s) |
ka-fujita@aist.go.jp
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| Organization name |
National Institute of Advanced Industrial Science and Technology
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| Department |
Research Institute of Science for Safety and Sustainability
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| Street address |
16-1 Onogawa
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| City |
Tsukuba |
| ZIP/Postal code |
3058569 |
| Country |
Japan |
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| Platforms (1) |
| GPL7294 |
Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA564274 |
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