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Series GSE13699 Query DataSets for GSE13699
Status Public on Dec 01, 2008
Title Immune response to the yellow fever vaccine 17D.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Correlates of immune mediated protection to most viral and cancer vaccines are still unknown. This impedes the development of novel vaccines to incurable diseases such as HIV and cancer. In this study, we have used functional genomics and polychromatic flow cytometry to define the signature of the immune response to the yellow fever (YF) vaccine 17D (YF17D) in a cohort of forty volunteers followed for up to one year after vaccination. We show that immunization with YF17D leads to an integrated immune response that includes several effector arms of innate immunity including complement, the inflammasome and interferons, as well as adaptive immunity as shown by an early T cell response followed by a brisk and variable B cell response. Development of these responses is preceded, as demonstrated in three independent vaccination trials and in a novel in vitro system of primary immune responses (Modular IMmune In vitro Construct (MIMIC) system), by the coordinated up-regulation of transcripts for specific transcription factors including STAT1, IRF7 and ETS2 that are upstream of the different effector arms of the immune response. These results clearly show that the immune response to a strong vaccine is preceded by coordinated induction of masters transcription factors, that lead to the development of a broad, polyfunctional and persistent immune response that integrates all effector cells of the immune system.
 
Overall design This study has 3 branches, two of which are in vivo vaccination studies in two different Locations (Montreal,Canada and Lausanne, Switzerland). The third branch is an in vitro system mimicing (VaxDesign Mimic experiment) the environment in a lymph node. The Montreal Cohort enrolled 15 donors, and sampled whole blood pre vaccination with the Sanofi-Pasteur YF17D-204 YF-VAX vaccine, and on days 3,7,10,14,28,60,365 post vaccination. In the Lausanne Cohort 11 donors were enrolled vaccinated with Stamail, wohle blood was sampled on days 0,3 and 7. The VaxDesign experiment used purified CD4+ T-cells and autologus DC from 3 different donors, YF17D stimulated and unstimulated control samples were analyzed on day 0,3 and 7 after the start of co culturing of T-Cells and DC.
 
Contributor(s) Gaucher D, Therrien R, Boucher G, Kettaf N, Angermann BR, Filali-Mouhim A, Moser JM, Mehta RS, Drake III DR, Castro E, Akondy R, Rinfret A, Yassine-Diab B, Said EA, Chouikh Y, Cameron MJ, Clum R, Kelvin D, Somogyi R, Greller LD, Balderas RS, Wilkinson P, Pantaleo G, Tartaglia J, Haddad EK, Sekaly R
Citation(s) 19047440
Submission date Nov 20, 2008
Last update date Mar 20, 2017
Contact name Bastian Robert Angermann
E-mail angerb@gmx.de
Fax 5143437854
Organization name Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CR-CHUM) Saint-Luc
Lab Laboratoire d'immunologie
Street address 264 René-Lévesque Est
City Montréal
State/province Quebec
ZIP/Postal code H2X 1P1
Country Canada
 
Platforms (2)
GPL6104 Illumina humanRef-8 v2.0 expression beadchip
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (142)
GSM343922 YF015_NS_14; Montreal Cohort
GSM343923 YF016_NS_3; Montreal Cohort
GSM343924 YF016_NS_7; Montreal Cohort
Relations
BioProject PRJNA109425

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13699_RAW.tar 7.4 Mb (http)(custom) TAR
SRA Run SelectorHelp
Raw data included within Sample table
Processed data included within Sample table

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