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Status |
Public on Feb 20, 2020 |
Title |
Cellular microRNAs in Huh7.5.1 cells |
Organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
Small RNAs play a critical role in host-pathogen interaction and microRNAs have emerged as key regulators of viral infections. Based on this evidence, we wished to contribute to this research field by identifying and characterizing cellular microRNAs with a positive or negative role on Sindbis virus (SINV) infection.
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Overall design |
Taking advantage of large scale sequencing, we cloned and sequenced small RNAs from Huh7.5.1 cells. We analyzed the expression levels of cellular microRNAs and we compared with the results of a mimic genome-wide screening using SINV-GFP as reporter. Altogether, our results indicate a positive role for the cellular miR-124-3p in SINV infection.
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Contributor(s) |
López P, Girardi E, Mounce BC, Weiss A, Chane-Woon-Ming B, Messmer M, Kaukinen P, Kopp A, Bortolamiol-Becet D, Fendri A, Vignuzzi M, Brino L, Pfeffer S |
Citation(s) |
32102877 |
Submission date |
Sep 03, 2019 |
Last update date |
May 21, 2020 |
Contact name |
Sébastien Pfeffer |
Organization name |
CNRS - Institut de Biologie Moléculaire et Cellulaire (IBMC)
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Department |
UPR 9002 - Architecture et Réactivité de l'ARN
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Lab |
RNA regulation in viral infections
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Street address |
2 allée Konrad Roentgen
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City |
Strasbourg |
ZIP/Postal code |
67084 |
Country |
France |
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Platforms (1) |
GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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Samples (1) |
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Relations |
BioProject |
PRJNA563554 |
SRA |
SRP220101 |