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Series GSE136740 Query DataSets for GSE136740
Status Public on Feb 20, 2020
Title Cellular microRNAs in Huh7.5.1 cells
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Small RNAs play a critical role in host-pathogen interaction and microRNAs have emerged as key regulators of viral infections. Based on this evidence, we wished to contribute to this research field by identifying and characterizing cellular microRNAs with a positive or negative role on Sindbis virus (SINV) infection.
 
Overall design Taking advantage of large scale sequencing, we cloned and sequenced small RNAs from Huh7.5.1 cells. We analyzed the expression levels of cellular microRNAs and we compared with the results of a mimic genome-wide screening using SINV-GFP as reporter. Altogether, our results indicate a positive role for the cellular miR-124-3p in SINV infection.
 
Contributor(s) López P, Girardi E, Mounce BC, Weiss A, Chane-Woon-Ming B, Messmer M, Kaukinen P, Kopp A, Bortolamiol-Becet D, Fendri A, Vignuzzi M, Brino L, Pfeffer S
Citation(s) 32102877
Submission date Sep 03, 2019
Last update date May 21, 2020
Contact name Sébastien Pfeffer
Organization name CNRS - Institut de Biologie Moléculaire et Cellulaire (IBMC)
Department UPR 9002 - Architecture et Réactivité de l'ARN
Lab RNA regulation in viral infections
Street address 2 allée Konrad Roentgen
City Strasbourg
ZIP/Postal code 67084
Country France
 
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (1)
GSM4056958 sRNAs from Huh7.5.1 cells
Relations
BioProject PRJNA563554
SRA SRP220101

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136740_RAW.tar 10.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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