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| Status |
Public on Aug 26, 2022 |
| Title |
Evaluation of Genome-wide chromatin accessibility of BM T cell subsets [ATAC_TC] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Chromatin accessibility of bone marrow conventional T cells, pTregs, tTregs, BMAC-induced Tregs and in vitro-induced Tregs were compared
Bone marrow (BM) harbors a large repertoire of regulatory T (Treg) cells, which are essential in hematopoiesis and peripheral tolerance. Treg cell accumulation in BM has been viewed as a consequence of preferential immigration of thymus-derived Treg cells. Here we report a novel subset of antigen presenting cells, which expresses the autoimmune regulator (Aire). These BM Aire-expressing cells resemble a subset of CD138+B220-TACI+Blimp-1+MHC-II+ plasma cells (pc-BMACs) and express a diverse repertoire of tissue-restricted self-antigens. pc-BMACs present self-antigens to na ve CD4+ T cells and can efficiently convert these into functionally competent CD25+Foxp3+ bona fide peripheral Treg cells (pTregs) capable of exerting immune suppression in vitro and in vivo. Aire expression may contribute to the tolerogenic function of pc-BMACs as it regulates the expression of genes involved in pTreg induction and function, including Icosl and retinoic acid synthesis-related genes, Aldh1a2 and Aldh1b1. Our data thus demonstrate an Aire-expressing plasma cell subset in the BM capable to promote peripheral tolerance by ectopically expressing tissue-restricted self-antigens and generating pTregs.
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| Overall design |
6.5TCR (HA-specific CD4) T cells were transferred into Aire-HA host and bone marrow T cell subsets were sorted 14 days post transfer for comparison of their chromatin accessibility
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| Contributor(s) |
Chen C, Schmidl C, Riegel D, Durst F, Beckhove P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 29, 2019 |
| Last update date |
Aug 28, 2022 |
| Contact name |
Philipp Beckhove |
| E-mail(s) |
philipp.beckhove@rcii.de
|
| Organization name |
Regensburg Center for Interventional Immunology
|
| Street address |
Franz-Josef-Strauss-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93053 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (12)
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| GSM4054535 |
Donor 6.5TCR T cells which were not converted to Tregs, rep1 |
| GSM4054536 |
Donor 6.5TCR T cells which were not converted to Tregs, rep2 |
| GSM4054537 |
Donor 6.5TCR T cells which were converted to Tregs, rep1 |
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| This SubSeries is part of SuperSeries: |
| GSE136650 |
Induction of autoreactive regulatory T cells through promiscuous gene expression by bone marrow-resident Aire+ antigen-presenting cells |
|
| Relations |
| BioProject |
PRJNA562923 |
| SRA |
SRP219846 |
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