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| Status |
Public on Aug 30, 2019 |
| Title |
Histone concentration times the cell cycle and transcription in early development. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study measures transcriptomic differences due to overexpression (via Slbp RNAi) or knockdown (via abo mutation) of histones in precisely staged Drosophila melanogaster embryos spanning the maternal to zygotic transition.
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| Overall design |
This study was conducted as 3 different RNA-seq experiments; a) Slbp v/s WT single embryo broad timecourse comparison b) abo v/s WT single time point pooled embryo comparison and c) abo v/s WT single embryo timecourse comparison For Slbp (single embryo) comparison: 5 replicates each of Slbp and WT pre-nuclear cycle (NC) 9 embryos; 4 replicates of Slbp and 6 replicates of WT NC13; 5 replicates of WT NC14 embryos. Slbp NC13 Replicates 1 and 2 and WT NC13 Replicate 1 were also sequenced multiple times i.e. technical replicates. For abo single time-point (40 pooled embryo) comparison: 3 replicates each of abo and WT at NC14. For abo timcourse (single embryo) comparison: 2 replicates of abo unfertilized (NC0) and 3 replicates of WT NC0; 3 replicates each of abo and WT at NC10, NC11, NC12, NC13 and NC14. The abo time course libraries were sequenced over 4 lanes in 2 different in different batches to get the necessary read depth i.e. technical replicates.
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| Contributor(s) |
Chari S, Amodeo AA |
| Citation(s) |
31511251 |
| Submission date |
Aug 29, 2019 |
| Last update date |
Jan 12, 2020 |
| Contact name |
Amanda Amodeo |
| E-mail(s) |
aamodeo@princeton.edu
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| Phone |
6092589406
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| Organization name |
Princeton University
|
| Department |
Lewis-Sigler Institute for Integrative Genomics
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| Street address |
Carl Ichan Laboratory, Washington Rd
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| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08544 |
| Country |
USA |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (175)
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| Relations |
| BioProject |
PRJNA562896 |
| SRA |
SRP219644 |
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