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| Status |
Public on Aug 30, 2019 |
| Title |
Akap11 is a positive regulator of osteogenesis in mouse osteoblastic cell line MC3T3-E1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: This is a post-GWAS functional study that aims to determine the effect of Akap11 knockout in mouse preosteoblastic cell line MC3T3-E1 during osteogenesis.
Methods: mRNA profile of WT (treated with CRISPR and remains unedited) and Akap11 knockout MC3T3-E1 cells were generated by RNAseq from pooled RNA (from 3 biological triplicate at equal quantity) of various timepoint during osteogenesis (Day 0, 7,16, 25).100ng total RNA was used for library construction by KAPA Stranded mRNA-Seq Kit (Roche). Pair-End 101bp sequencing was performed in the Illumin HiSq1500 sequencer using the HiSeq SBS Kit v4 (Illumina). Data was collected with SCS version 1.4.8. Base calling was performed using Illumina’s RTA (Version 1.12.4.2). 7 samples were sequenced per lane and achieved a sequencing depth of ~65 million read per sample.
Results: Expression of osteogentic markers in Akap11 knockout MC3T3-E1 cells were suppressed. Genes invovled in IGF-I signaling pathway were signficantly altered.
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| Overall design |
mRNA profile of Day0, 7, 16, 25 WT (treated with CRISPR and remains unedited) and Akap11 knockout MC3T3-E1 cells were generated by pooled PolyA mRNA Sequencing .
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| Contributor(s) |
Li GH, Cheng VK, Cheung CL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 29, 2019 |
| Last update date |
Aug 31, 2019 |
| Contact name |
Vincent Ka Fai Cheng |
| E-mail(s) |
u3003930@connect.hku.hk
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| Phone |
67383950
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| Organization name |
The University of Hong Kong
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| Department |
Pharmacology and Pharmacy
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| Street address |
L202 lab block 21 Sassoon road Pokfulam Hong Kong
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| City |
Hong Kong |
| State/province |
-- select -- |
| ZIP/Postal code |
000 |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA562822 |
| SRA |
SRP219825 |
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