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Series GSE135983 Query DataSets for GSE135983
Status Public on May 14, 2020
Title Integrin α3β1-dependent mRNA expression profile of hair bulge keratinocytes and their progeny during DMBA/TPA-mediated skin tumorigenesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Integrin α3β1 in hair bulge population affects the tumor formation following DMBA/TPA carcinogenesis protocol, however linage tracing showed that hair bulge cells do not largely contributre to tumor mass. To investigate whether α3β1 might affect tumor promoting environment and to better understand its role in hair bulge population during skin tumorigenesis, we determined the expresison profile of hair bulge-originating keratinocytes isolted from α3β1KO and WT mice during initiation of tumorigenesis.
Overall design CRE-induced GFP-positive keratinoctes from K19(Cre-ERT); mTmG; Itga3 fl/fl and K19(Cre-ERT); mTmG mice, submitted to short-term DMBA/TPA treatment, were isolated using FACS and their expression profile was determined using next generation RNA sequencing
Contributor(s) Ramovs V, Sonnenberg A, de Rink I
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Submission date Aug 19, 2019
Last update date May 14, 2020
Contact name Veronika Ramovs
Organization name Netherlands Cancer Institute
Department Cell Biology
Lab A. Sonnenberg
Street address Plesmanlaan 121
City Amsterdam
ZIP/Postal code 1066 CX
Country Netherlands
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM4038936 WT sample 1
GSM4038937 WT sample 2
GSM4038938 WT sample 3
BioProject PRJNA560882
SRA SRP218806

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Supplementary file Size Download File type/resource
GSE135983_genecounts.txt.gz 1.0 Mb (ftp)(http) TXT
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