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| Status |
Public on Aug 14, 2019 |
| Title |
RNASeq of bone lining niche cell components from myeloma-bearing mice treated daily with small molecule LDN193189 (LDN) or vehicle control |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We compare gene expression in FACS-sorted bone lining cell components (stromal progenitors, osteoprogenitors, endothelial cells) from LDN193189 vs. vehicle-treated myeloma-bearing mice, and in myeloma cells flushed from the central marrow vs. myeloma cells digested from the bone-lining niche. The myeloma model used is the murine 5TGM1 cell line injected into immunocompetent KaLwRij mice. LDN193189 is a type 1 BMP receptor inhibitor.
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| |
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| Overall design |
Comparative gene expression differences in 100-cell aliquots of sorted bone marrow niche or myeloma cell types. N = 4 - 8 biological replicates per cell type (from different mice) per condition (LDN or vehicle treated).
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| |
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| Contributor(s) |
Gooding S, Repapi E |
| Citation(s) |
31586071 |
| |
| Submission date |
Aug 13, 2019 |
| Last update date |
Oct 15, 2019 |
| Contact name |
Sarah Gooding |
| E-mail(s) |
sarah.gooding@rdm.ox.ac.uk
|
| Phone |
00441865222410
|
| Organization name |
Oxford University
|
| Department |
Weatherall Institute of Molecular Medicine
|
| Lab |
Drakesmith Lab
|
| Street address |
John Radcliffe Site, Headley Way
|
| City |
Oxford |
| State/province |
Oxon |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (58)
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| This SubSeries is part of SuperSeries: |
| GSE135786 |
Transcriptomic profiling of the myeloma bone-lining niche identifies BMP signalling as a target for bone disease |
|
| Relations |
| BioProject |
PRJNA560057 |
| SRA |
SRP218239 |
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