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| Status |
Public on Oct 01, 2019 |
| Title |
Single Cell RNA Sequencing Analysis of Polystyrene Microplastic Exposed and control Zebrafish Intestine |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Microplastics (MPs) as widespread contamination pose high risk for aquatic organisms. However, current understanding of MP toxicities are based on cell population-averaged measurements. Here we used single-cell RNA sequencing to provide the transcriptome heterogeneity of 12000 intestinal cells obtained from zebrafishes exposed to 100nm, 5μm and 200μm polystyrene MPs (PS-MPs) for 21 days. Eight intestinal cell populations were identified. We found that all the three sizes of PS-MPs induced dysfunction of intestinal immune cells (including phagosome and regulation of immune system process).
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| |
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| Overall design |
Single cell RNA sequencing were adopted to learn the transcriptional variation in zebrafish intestine followed exposure MPs with different size (100 nm, 5 μm and 200 μm).
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| |
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| Contributor(s) |
Wu B, Gu W |
| Citation(s) |
32092251 |
| |
| Submission date |
Aug 13, 2019 |
| Last update date |
Mar 01, 2020 |
| Contact name |
Gu Weiqing |
| E-mail(s) |
gwq960722@sina.com
|
| Phone |
13962113111
|
| Organization name |
Nanjing University
|
| Street address |
Xianlin
|
| City |
Nanjing |
| ZIP/Postal code |
210023 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24995 |
Illumina NovaSeq 6000 (Danio rerio) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE136109 |
Single Cell RNA Sequencing and Intestinal Microbial Analysis of Polystyrene Microplastic Exposed Zebrafish Intestine |
|
| Relations |
| BioProject |
PRJNA560029 |
| SRA |
SRP218210 |
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