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Series GSE135766 Query DataSets for GSE135766
Status Public on Dec 11, 2019
Title The chaperone FACT and H2B ubiquitination maintain S. pombe genome architecture through genic and subtelomeric functions [ChIP-Seq]
Organism Schizosaccharomyces pombe
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The histone chaperone FACT and histone H2B ubiquitination (H2Bub) facilitate RNA Polymerase II passage through chromatin. It remains unknown to what extent they cooperate in vivo. Here, we used genome-wide, genetic, biochemical and microscopic approaches to dissect their interplay in the fission yeast Schizosaccharomyces pombe. We show that FACT and H2Bub repress antisense transcripts near the 5’ end and inside gene bodies, respectively. The accumulation of these transcripts accompanies with changes at genic nucleosomes in FACT and H2Bub mutants. Moreover, in the H2Bub mutant, FACT binding to histones is stabilized. Interestingly, analysis of FACT-H2Bub double mutants revealed that FACT depletion suppresses genic nucleosome loss in the H2Bub mutant. In addition, FACT, unlike H2Bub, maintains nucleosomes in intergenic regions. This is especially important for subtelomeric gene repression and in compacting chromatin into ‘knobs’. Collectively, our study demonstrates that FACT and H2Bub functionally interact with each other in a genome-context dependent manner. Further, our data indicate an involvement of FACT in higher order chromatin formation outside of euchromatin.
 
Overall design ChIP-seq experiments in wild type, H2BK119R, pob3 and double mutant fission yeast.
 
Contributor(s) Murawska M, Schauer T, Ladurner AG
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Submission date Aug 13, 2019
Last update date Dec 12, 2019
Contact name Tamas Schauer
E-mail(s) tamas.schauer@med.uni-muenchen.de
Organization name LMU Biomedical Center
Department Molecular Biology
Lab Bioinformatics
Street address Großhaderner Strasse 9
City Planegg-Martinsried
ZIP/Postal code 82152
Country Germany
 
Platforms (1)
GPL13988 Illumina HiSeq 2000 (Schizosaccharomyces pombe)
Samples (44)
GSM4029348 H2Bub_pob3_1
GSM4029349 H2Bub_pob3_2
GSM4029350 H2Bub_WT_1
This SubSeries is part of SuperSeries:
GSE124092 The chaperone FACT and H2B ubiquitination maintain S. pombe genome architecture through genic and subtelomeric functions
Relations
BioProject PRJNA560030
SRA SRP218209

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135766_RAW.tar 2.0 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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