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| Status |
Public on Jul 13, 2020 |
| Title |
High- and low-protein diet: effects on human hepatic fat content, autophagy, mitochondrial function and fat metabolism |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Non-alcoholic fatty liver disease (NAFLD) is becoming increasingly prevalent and nutrition intervention remains the most important therapeutic approach for NAFLD. Our aim was to investigate whether low- (LP) or high-protein (HP) diets are more effective in reducing liver fat and reversing NAFLD. Here RNA-seq analysis was used to analyse which metabolic pathways that were altered on the LP and HP diets.
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| Overall design |
19 morbidly obese subjects undergoing bariatric surgery were randomized into two hypocaloric (1500 kcal/day) diet groups, a low protein (LP: 10 E% protein, n=10) and a high protein (HP: 30 E% protein, n=9), for three weeks prior to surgery. RNA-seq analyses were performed on liver samples collected during surgery.
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| Contributor(s) |
Xu C, Markova M, Loft A, Herzig S, Pivovarova-Ramich O, Pfeiffer AF |
| Citation(s) |
32652799 |
| |
| Submission date |
Aug 06, 2019 |
| Last update date |
Jul 13, 2020 |
| Contact name |
Anne Loft |
| E-mail(s) |
anlo@bmb.sdu.dk
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| Organization name |
University of Southern Denmark
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| Department |
Biochemistry & Molecular Biology
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| Street address |
Campusvej 55
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| City |
Odense M |
| ZIP/Postal code |
5230 |
| Country |
Denmark |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA558947 |
| SRA |
SRP217536 |
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