|
| Status |
Public on Aug 01, 2020 |
| Title |
Streamlined low-input transcriptomics through EASY-RNAseq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
we describe a streamlined RNAseq protocol (EASY RNAseq) for sensitive transcriptome assessment starting from low amount of input materials. EASY RNAseq is technically robust enough for sequencing small pools of homogenous and heterogeneous cells, recovering higher numbers of genes and with a more even expression distribution pattern than other commonly used methods.
|
| |
|
| Overall design |
To evaluate the capbility of EASY-RNASeq respect to the amount of input materials, different amount of input materials extracted from mice spleen(0.1ng, 0.5ng, 1.0ng, 5.0ng) were used for constructing EASY-RNASeq libraries. And for cells sample, different number of input cells obtained from murine CD3+ T cells(1e2, 5e2, 1e3, 1e4) were used to do EASY-RNASeq library construction. All libraries were sequenced by Illumina platform.
|
| |
|
| Contributor(s) |
Zhou Y, Xu H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA542941 |
| |
| Submission date |
Aug 02, 2019 |
| Last update date |
Aug 01, 2020 |
| Contact name |
peng zhou |
| E-mail(s) |
zhoupeng@tcrcure.com
|
| Organization name |
First Affiliated Hospital of Army Medical University
|
| Street address |
重庆市大渡口区天安数码城5栋10层
|
| City |
chongqing |
| ZIP/Postal code |
400000 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (23)
|
|
| Relations |
| SRA |
SRP198420 |
Less...
More...