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| Status |
Public on Jul 16, 2021 |
| Title |
RNA profiling of human lymphatic endothelial cells exposed to melanoma exosomes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In order to study the influence of melanoma-derived exosomes in the lymphatic vasculature within the lymph nodes, we have profile the transcriptional changes occurring in human lymphatic endothelial cells treated with melanoma-secreted exosomes or with the conditioned medium from melanoma cells depleted of exosomes.
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| |
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| Overall design |
Human lymphatic endothelial cells (HMVECs) were treated for 24h and 48h with exosomes from SK-MEL-147 melanoma cell line and conditioned medium from the same line depleted of exosomes. RNA was extracted with trizol and subsequent Qiagen columns. RNA sequencing was performed afterwards.
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| Web link |
https://doi.org/10.1038/s43018-021-00272-y
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| |
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| Contributor(s) |
Silva SG, Graña O, Peinado H |
| Citation missing |
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| |
| Submission date |
Jul 31, 2019 |
| Last update date |
Dec 14, 2021 |
| Contact name |
Osvaldo Graña |
| Organization name |
CNIO
|
| Department |
Structural Biology
|
| Lab |
Bioinformatics Unit
|
| Street address |
C/ Melchor Fernández Almagro, 3
|
| City |
Madrid |
| State/province |
Comunidad de Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
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| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA557684 |
| SRA |
SRP216946 |
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