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| Status |
Public on Jul 30, 2019 |
| Title |
RNA-Seq for identification of differntially expressed genes by VxrB(D78E) overexpression |
| Organism |
Vibrio cholerae O1 biovar El Tor str. N16961 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Bactericidal antibiotics are powerful drugs because they not only inhibit essential bacterial functions, but convert them into toxic processes. Many bacteria are remarkably tolerant against antibiotics, due to inducible damage repair responses. How these responses promote whole population tolerance in important human pathogens is poorly understood. The two-component system VxrAB of the diarrheal pathogen Vibrio cholerae, a model system for tolerance against cell wall damaging (e.g., beta-lactam) antibiotics, is required for high-level beta-lactam tolerance. Here, we report the mechanism of VxrAB-mediated survival. We find that -lactam antibiotics inappropriately induce the Fur-regulated iron starvation response, causing an increase in intracellular free iron and colateral oxidative damage. VxrAB reduces antibiotic-induced toxic influx of Fe by downregulating iron importers and induces cell wall synthesis functions to counteract cell wall damage. Our results highlight the complex responses elicited by antibiotics and suggest that the ability to counteract diverse stresses promotes high-level antibiotic tolerance.
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| Overall design |
To identify controlled genes by VxrB, we carried out RNA-Seq analysis with wild type and VxrB(D78E) overexpressed samples.
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| Contributor(s) |
Shin J, Doerr T |
| Citation missing |
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| |
| Submission date |
Jul 29, 2019 |
| Last update date |
Aug 02, 2019 |
| Contact name |
JUNG HO Cornell SHIN |
| E-mail(s) |
jhs0421@gmail.com
|
| Phone |
6077935562
|
| Organization name |
Weill Institute at Cornell University
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| Department |
Microbiology
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| Lab |
Tobias Doerr
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| Street address |
363 Weill Hall, Cornell University
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
| GPL26987 |
Illumina HiSeq 2500 (Vibrio cholerae O1 biovar El Tor str. N16961) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA557133 |
| SRA |
SRP216661 |
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