|
| Status |
Public on Jul 26, 2019 |
| Title |
CHO_library_B_GCN Screen |
| Organisms |
Cricetulus griseus; synthetic construct |
| Experiment type |
Other
|
| Summary |
CRISPR screen targeting 3022 CHO (Chinese hamster) genes to identify those contributing to induction of the Integrated Stress Response by histidinol treatment.
|
| |
|
| Overall design |
FACS sorting was used to select CHO cells that failed to induce a CHOP::GFP Integrated Stress Response (ISR) reporter following targeting with a focused CRISPR mutagenesis library.
|
| |
|
| Contributor(s) |
Harding HP, Ordonez A, Allen F, Parts L, Ingles AJ, Williams RL, Ron D |
| Citation(s) |
31749445 |
| |
| Submission date |
Jul 25, 2019 |
| Last update date |
Nov 22, 2019 |
| Contact name |
Heather P Harding |
| E-mail(s) |
hph23@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
CIMR Clinical Biochemistry
|
| Street address |
Hills Road
|
| City |
Cambridge |
| State/province |
Cambridge |
| ZIP/Postal code |
CB2 0XY |
| Country |
United Kingdom |
| |
|
| Platforms (3) |
| GPL26967 |
Illumina MiniSeq (synthetic construct) |
| GPL26968 |
Illumina MiniSeq (Cricetulus griseus) |
| GPL26969 |
Illumina HiSeq 4000 (Cricetulus griseus) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA556755 |
| SRA |
SRP216457 |
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