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Series GSE134826 Query DataSets for GSE134826
Status Public on Dec 15, 2019
Title A Central Role for PRC1 in Shaping the 3D Nuclear Landscape
Organism Mus musculus
Experiment type Other
Summary 1. Loss of RING1B substantially disrupts nuclear architecture.
2. PRC1 mediated looping can occur at a Mb scale and is independent of CTCF.
3. Polycomb mediated looping is driven by canonical PRC1 complexes.
4. Trimeric PRC1-mediated interactions occur in vitro and in vivo.
5. Gene up-regulation does not directly lead to the loss of PRC1 loops.
 
Overall design Hi-C analysis of WT, RING1B I53A and RING1B KO E14 mouse ES cells; 4 replicates for WT and 2 replicates each, for both mutants.
 
Contributor(s) Flyamer IM, Boyle S, Williamson I, Sengupta D, Bickmore WA, Illingworth RS
Citation(s) 32439634
Submission date Jul 25, 2019
Last update date Jun 10, 2020
Contact name Ilya M. Flyamer
E-mail(s) flyamer@gmail.com
Organization name Friedrich Miescher Institute for Biomedical Research
Lab Giorgetti
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (8)
GSM3972588 E14 WT 1
GSM3972589 E14 WT 2
GSM3972590 E14 WT 3
Relations
BioProject PRJNA556587
SRA SRP216335

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134826_I53A.mm9.mapq_30.1000.mcool 2.0 Gb (ftp)(http) MCOOL
GSE134826_KO.mm9.mapq_30.1000.mcool 2.3 Gb (ftp)(http) MCOOL
GSE134826_RAW.tar 37.4 Gb (http)(custom) TAR (of MCOOL, PAIRS, TXT)
GSE134826_WT.mm9.mapq_30.1000.mcool 2.0 Gb (ftp)(http) MCOOL
GSE134826_stats.tsv.gz 16.9 Kb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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