|
Status |
Public on Dec 15, 2019 |
Title |
A Central Role for PRC1 in Shaping the 3D Nuclear Landscape |
Organism |
Mus musculus |
Experiment type |
Other
|
Summary |
1. Loss of RING1B substantially disrupts nuclear architecture. 2. PRC1 mediated looping can occur at a Mb scale and is independent of CTCF. 3. Polycomb mediated looping is driven by canonical PRC1 complexes. 4. Trimeric PRC1-mediated interactions occur in vitro and in vivo. 5. Gene up-regulation does not directly lead to the loss of PRC1 loops.
|
|
|
Overall design |
Hi-C analysis of WT, RING1B I53A and RING1B KO E14 mouse ES cells; 4 replicates for WT and 2 replicates each, for both mutants.
|
|
|
Contributor(s) |
Flyamer IM, Boyle S, Williamson I, Sengupta D, Bickmore WA, Illingworth RS |
Citation(s) |
32439634 |
Submission date |
Jul 25, 2019 |
Last update date |
Jun 10, 2020 |
Contact name |
Ilya M. Flyamer |
E-mail(s) |
flyamer@gmail.com
|
Organization name |
Friedrich Miescher Institute for Biomedical Research
|
Lab |
Giorgetti
|
Street address |
Maulbeerstrasse 66
|
City |
Basel |
ZIP/Postal code |
4058 |
Country |
Switzerland |
|
|
Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
Samples (8)
|
|
Relations |
BioProject |
PRJNA556587 |
SRA |
SRP216335 |