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Series GSE134774 Query DataSets for GSE134774
Status Public on Jan 04, 2020
Title mSeq of total and polysome bound mRNAs in two yeast strains
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary We report on how the association of mRNA to translating ribosomes changes due to the absence of the expansion segment 27L in S. cerevisiae. We observe specific enrichment and depletion of mRNA sets from rmutant ribosomes which reshapes the proteome.
Overall design Examination of total and ribosome associated mRNA in wild-type and Δ27Lb yeast yJD1045 strain depleted of genome rDNA. rRNA is exclusively plasmid borne.
Contributor(s) Shankar V, Rauscher R, Gharib WH, Reuther J, Koch M, Polacek N
Citation(s) 31960048
Submission date Jul 24, 2019
Last update date Apr 08, 2020
Contact name Norbert Polacek
Organization name University of Bern
Department Chemistry and Biochemistry
Street address Freiestrasse 3
City Bern
ZIP/Postal code 3012
Country Switzerland
Platforms (1)
GPL23014 Illumina HiSeq 3000 (Saccharomyces cerevisiae)
Samples (12)
GSM3968534 WT_total mRNA_rep1
GSM3968535 WT_total mRNA_rep2
GSM3968536 WT_total mRNA_rep3
BioProject PRJNA556393
SRA SRP216211

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Supplementary file Size Download File type/resource
GSE134774_RAW.tar 400.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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