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Series GSE134584 Query DataSets for GSE134584
Status Public on Jul 20, 2019
Title Circular RNA Expression Profiles in the Plasma from Patients with Heart Failure
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary To identify the circRNA expression profiles in HF patients’ plasma and to evaluate the potential application of circRNAs for HF diagnosis, circRNA microarrays were performed on plasma samples obtained from HF patients and healthy controls. The RNAs of the plasma from the HF and control groups were extracted for microarray analysis. The purified RNAs were hybridized to a microarray (Agilent human circRNA Array V2.0) containing 170,340 human circRNA probes. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to validate the results.
 
Overall design Peripheral blood samples were collected from three HF patients and three control groups into blood collection tubes containing 85 mM trisodium citrate, 78 mM citric acid, and 111 mM glucose. The whole blood was centrifuged at 2000 × g for 10 min at 22 °C to obtain plasma. Plasma was transferred to a new tube and stored at –80 °C in aliquots.
 
Contributor(s) Sun Y, Jiang X
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Submission date Jul 19, 2019
Last update date Jul 24, 2019
Contact name yeying sun
E-mail(s) sunyy21cn@163.com
Organization name Binzhou Medical Univeristy
Street address No.346 Guanhai road
City Yantai
ZIP/Postal code 264003
Country China
 
Platforms (1)
GPL26925 Agilent-084217 CapitalBio Technology Human CircRNA Array v2
Samples (6)
GSM3956449 HF plasma sample1
GSM3956450 HF plasma sample2
GSM3956451 HF plasma sample3
Relations
BioProject PRJNA555656

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134584_RAW.tar 38.7 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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