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| Status |
Public on Jul 17, 2023 |
| Title |
Expression data from white matter astrocytes of wild-type or GnT-IX-deficient mice after cuprizone administration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Multiple sclerosis (MS) is an immune-based demyelinating disease. Currently available therapeutics target inflammation but have little impact on promoting remyelination. Furthermore, MS diagnosis is sometimes challenging. Using a demyelination model mice, we previously found that protein tyrosine phosphatase receptor type zeta (PTPRZ) receives abnormal glycosylation, a branched O-mannosyl (O-Man) glycan and that branched O-Man glycosylated PTPRZ specifically occurs in reactive astrocytes of demyelinated lesions. Furthermore, by genetically deleting the branching enzyme, GnT-IX (also known as GnT-Vb), astrogliosis was attenuated and remyelination enhanced. To characterize the PTPRZ expressing astrocytes, microarray analysis was performed using astrocytes from mice after short and long term-cuprizone administration.
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| Overall design |
Astrocytes were isolated from white matters of wild-type and GnT-IX-deficient mice after 0, 3, or 12 weeks of cuprizone administration and used for microarray analysis. Three independent biological replicates were performed.
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| Contributor(s) |
Kitazume S, Sakuda K |
| Citation(s) |
37005741 |
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| Submission date |
Jul 16, 2019 |
| Last update date |
Oct 16, 2023 |
| Contact name |
Shinobu Kitazume |
| E-mail(s) |
shinobuk@fmu.ac.jp
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| Phone |
81-24-547-1958
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| Organization name |
Fukushima Medical University
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| Street address |
1 Hikariga-oka
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| City |
Fukushima City |
| State/province |
Fukushima |
| ZIP/Postal code |
960-1295 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (12)
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| GSM3944028 |
wild-type_cuprizone 0 weeks, biological rep 1 |
| GSM3944029 |
wild-type_cuprizone 0 weeks, biological rep 2 |
| GSM3944030 |
wild-type_cuprizone 0 weeks, biological rep 3 |
| GSM3944031 |
wild-type_cuprizone 3 weeks, biological rep 1 |
| GSM3944032 |
wild-type_cuprizone 3 weeks, biological rep 2 |
| GSM3944033 |
wild-type_cuprizone 3 weeks, biological rep 3 |
| GSM3944034 |
wild-type_cuprizone 12 weeks, biological rep 1 |
| GSM3944035 |
wild-type_cuprizone 12 weeks, biological rep 2 |
| GSM3944036 |
wild-type_cuprizone 12 weeks, biological rep 3 |
| GSM3944037 |
GnT-IX-deficient_cuprizone 12 weeks, biological rep 1 |
| GSM3944038 |
GnT-IX-deficient_cuprizone 12 weeks, biological rep 2 |
| GSM3944039 |
GnT-IX-deficient_cuprizone 12 weeks, biological rep 3 |
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| Relations |
| BioProject |
PRJNA554889 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE134372_RAW.tar |
12.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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